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Development and validation of a microfluidic multiplex immunoassay for the determination of levels and avidity of serum antibodies to tetanus, diphtheria and pertussis antigens.
the microfluidic channels of the assay cartridges of the system. The developed assay showed very good sensitivity, specificity, and intra- and inter-assay coefficients of variation (CV s for the ...
Importance of anti-SARS-CoV-2 assay antigenic composition as revealed by the results of the Belgian external quality assessment (EQA) scheme.
Humans Immunoglobulin A Immunoglobulin G Immunoglobulin M Male Phosphoproteins SARS-CoV-2 Sensitivity and Specificity Spike Glycoprotein, Coronavirus Abstract: We report on sample IS /17575 since it ...
Measuring disability-adjusted life years (DALYs) due to COVID-19 in Scotland, 2020
certificates. We calculated DALY s based on the COVID-19 consensus model and methods outlined by the European Burden of Disease Network. DALY s were presented as a range, using a sensitivity analysis based on ...
Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing
metagenomics approach and Illumina sequencing. As anticipated, higher variation and lower sensitivity were observed at lower coverages and allelic frequencies. We found that detection of all low-frequency ...
Reliability of Morphological and PCR Methods for the Official Diagnosis of Aethina tumida (Coleoptera: Nitidulidae): A European Inter-Laboratory Comparison.
specificity. Sensitivity was satisfactory for all the participants and both types of methods, thus fully meeting the diagnostic challenge of confirming all truly positive cases as positive. Two participants ...
Retrospective evaluation of routine whole genome sequencing of Mycobacterium tuberculosis at the Belgian National Reference Center, 2019.
MTB strains analyzed in 2019 (n = 306). RESULTS: In all cases, the WGS-based procedure was able to identify correctly the MTB species. Compared to MGIT drug susceptibility testing (DST), the sensitivity ...
Strategy to Develop and Evaluate a Multiplex RT-ddPCR in Response to SARS-CoV-2 Genomic Evolution.
such as specificity, sensitivity and applicability. Therefore, a duplex reverse-transcription droplet digital PCR (RT-ddPCR) method was evaluated in silico by using 154 489 whole-genome sequences of ...
Quantitative decision making in animal health surveillance: Bovine Tuberculosis Surveillance in Belgium as case study.
used to simulate the current surveillance system’s expected performance in terms of detection sensitivity and specificity. These results were then descriptively compared with observed field results. ...
Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking
developed to target specifically the newly discovered GMM, namely GMM protease2. The performance of the real-time PCR marker was assessed in terms of specificity and sensitivity. The applicability of the ...
Validation of TaqMan-Based Assays for Specific Detection and Differentiation of Wild-Type and Neethling Vaccine Strains of LSDV.
DIVA qPCR assays able to distinguish Neethling-based LAV s and wild-type LSDV were developed. Upon validation, both assays were shown to have high sensitivity and specificity with a diagnostic ...