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Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
multilocus sequence typing and single nucleotide polymorphism analysis). Shiga toxin-producing Escherichia coli (STEC) was selected as a case study. Overall, data quality and inferred phylogenetic ...
Detection strategy targeting a chloramphenicol resistance gene from genetically modified bacteria in food and feed products
a chloramphenicol resistance gene, highlighted by the patent analysis performed in this study as being harboured by a noteworthy part of GMM producing microbial fermentation products from the food and feed industry. ...
Standardization of Somatic Variant Classifications in Solid and Haematological Tumours by a Two-Level Approach of Biological and Clinical Classes: An Initiative of the Belgian ComPerMed Expert Panel.
Sara Vander Borght; Els Van Valckenborgh; Aline Antoniou; Aline Hébrant Source: Cancers (Basel), Volume 11, Issue 12 (2019) Abstract: In most diagnostic laboratories, targeted next-generation sequencing ...
Validation of a Bioinformatics Workflow for Routine Analysis of Whole-Genome Sequencing Data and Related Challenges for Pathogen Typing in a European National Reference Center: as a Proof-of-Concept.
Keersmaecker; Nancy Roosens; Kevin Vanneste Source: Front Microbiol, Volume 10 (2019) Keywords: national reference center Neisseria meningitidis public health VALIDATION whole-genome sequencing Abstract: Despite ...
Comparative Analysis of Whole-Genome Sequence of African Swine Fever Virus Belgium 2018/1.
25, Issue 6 (2019) Abstract: We analyzed the whole-genome sequence of African swine fever virus Belgium 2018/1. The strain fits into the European genotype II (>99.98% identity). The high-coverage ...
Study of the underlying mechanisms and consequences of pathogenicity differences between two in vitro selected G1-H9N2 clones originating from a single isolate.
Influenza in Birds Poultry Diseases Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Viral Plaque Assay virulence Virus Shedding Abstract: The G1- H9N2 avian influenza virus (AIV) has caused ...
Orthogonal typing methods identify genetic diversity among Belgian Campylobacter jejuni strains isolated over a decade from poultry and cases of sporadic human illness.
Campylobacter jejuni Chickens Gastroenteritis Genetic Variation Humans Molecular Epidemiology Multilocus Sequence Typing polymerase chain reaction Poultry Abstract: Campylobacter jejuni is a zoonotic pathogen ...
Complete coding sequence of a novel picorna-like virus in a blackbird infected with Usutu virus.
Picornaviridae Infections Plants whole genome sequencing Abstract: Using random high-throughput RNA sequencing, the complete coding sequence of a novel picorna-like virus (a 9,228-nt contig containing 212,202 ...
Nucleotide Sequence Database Comparison for Routine Dermatophyte Identification by Internal Transcribed Spacer 2 Genetic Region DNA Barcoding.
to an ITS2 nucleotide sequence analysis. Sequences were analyzed against the databases, and the results were compared to clusters obtained via DNA alignment of sequence segments. The DNA tree served as ...
Complete Genome Sequence of an Avian Metapneumovirus Subtype A Strain Isolated from Chicken () in Brazil.
Trícia M F de S; Soares, Rodrigo M; Arns, Clarice W; Van Borm, Steven; Ferreira, Helena L Source: Genome Announc, Volume 5, Issue 29 (2017) Abstract: We report here the complete genome sequence of an avian ...