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Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR(R)Green Real-Time PCR Methods and High Resolution Melting Analysis418

shiga-toxin (stx1 and/or stx2) producing E. coli (VTEC or STEC respectively) have received a lot of attention recently. Indeed, due to the high number of outbreaks related to VTEC strains, the European Food ...

Survey of the contamination of foodstuffs of animal origin by Shiga toxin producing Escherichia coli serotype O157:H7 in Belgium from 1999 to 2003

survey Swine Universities university veterinary Abstract: A survey of the prevalence of Shiga toxin-producing Escherichia coli (STEC) of O157 serotype in foodstuffs of animal origin (beef, veal, pork, ...

Detection and characterization of verotoxigenic Escherichia coli by a VTEC/EHEC multiplex PCR in porcine faeces and pig carcass swabs36718

verotoxigenic-producing Escherichia coli, a sensitive and rapid isolation method was developed. A VTEC / EHEC multiplex PCR method was developed to test all samples taken at the farm (n=289) and at the slaughterhouse ...

Pathotypes of bovine verotoxigenic Escherichia coli isolates producing attaching/effacing (AE) lesions in the ligated intestinal loop assay in rabbits51

Common Diarrhea e Enterocytes Escherichia coli Escherichia coli Proteins Faculty function functions gene Genes Human Humans im in vivo Intestines IS journal Membrane Proteins microbiology Microvilli ...

Identification of eae sequences in enteropathogenic Escherichia coli strains from rabbits.

enteropathogenic Escherichia coli strains from rabbits. All of the strains produced diarrhea after experimental infection, attached to the brush borders of the intestinal lining, and possessed homology to the eae ...

Comparison of Droplet Digital PCR and qPCR for the Quantification of Shiga Toxin-Producing Escherichia coli in Bovine Feces.

toxin-producing Escherichia coli (STEC) and are often the direct or indirect source of STEC outbreaks in humans. Accurate measurement of the concentration of shed STEC in cattle feces could be a key answer to ...

Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.

virulence and antimicrobial resistance genotyping) and phylogenetic relatedness (core genome multilocus sequence typing and single nucleotide polymorphism analysis). Shiga toxin-producing Escherichia coli ...

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