Search results - 32 results

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

characterization had only been achieved in a handful of studies, and had not been proven possible when more than one strain of the same species were present. Moreover, the genome obtained from metagenomics sequences ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

detected, both at high and low percentages. No impact related to food processing or to the presence of other crop species was observed. The present proof-of-concept study has allowed us to deliver the first ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

contaminations consisted of GM   Bacillus  species belonging to the  B. subtilis  group. In order to screen for the potential presence of such contaminations, in this study we have developed a new real-time PCR ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

species in various concentrations and carrying AMR and/or virulence genes, taking the specific need of each application into account. Moreover, the short read and long read approaches are being compared to ...

Listeria monocytogenes isolates from Cornu aspersum snails: Whole genome-based characterization and host-pathogen interactions in a snail infection model.

reactive oxygen species (ROS) generation in snails’ haemolymph. In contrast, the administration of the non-virulent strain which possesses a truncated mogR gene that regulates flagellar motility gene ...

Sensitivity of candling as routine method for the detection and recovery of ascaridoids in commercial fish fillets.

make targeted recommendations to the fish industry, the Se was calculated per fish part, larval genus, and fish species. All fish parts (n = 615) were first candled, and larvae were collected, followed ...

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

presence of a GMM in a microbial fermentation product, with characterization based on the detection of AMR genes and vectors, species and unnatural associations in the GMM genome. In our proof‐of‐concept ...

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