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Development, evaluation and optimization of next generation sequencing data analysis tools in support of a fast response for public health and food chain safety

unauthorized GMO WGS-based subtyping Health Topics:  Health and disease monitoring Surveillance de la santé et des maladies Ziekten en gezondheid in kaart brengen Food consumption and food safety Consommation et ...

Comparison of SNP-based subtyping workflows for bacterial isolates using WGS data, applied to Salmonella enterica serotype Typhimurium and serotype 1,4,[5],12:i:.

Salmonella; Salmonella enterica; Salmonella typhimurium; SNP; subtyping; whole genome sequencing Abstract: Whole genome sequencing represents a promising new technology for subtyping of bacterial pathogens. ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

through a liquid bead suspension array in which the MOL- PCR products are hybridized to MagPlex- TAG beads.In this chapter, we describe the complete procedure for a MOL- PCR assay for subtyping of ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

detect multiple variants of the same allele, and (ii) significantly more cost-effective than commercial microarrays and WGS. The targeted β-lactamases include ESBL s (CTX-M families and subtypes, ESBL and ...

Data analysis associated with the use of whole genome sequencing for subtyping of pathogenic microorganisms, Salmonella Typhimurium as a case study

result results routine Salmonella Salmonella enterica Salmonella typhimurium Science situation SOCIAL Species Still study Subtyping subtyping of isolates Surveillance use WGS whole genome whole genome ...

Generation and Characterization of Six Recombinant Botulinum Neurotoxins as Reference Material to Serve in an International Proficiency Test.

40 subtypes. Expert laboratories currently use different technical approaches to detect, identify and quantify BoNT, but due to the lack of (certified) reference materials, analytical results can ...

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

resistance result results S Salmonella Salmonella enterica Salmonella typhimurium stability Subtyping Surveillance Technique Type use VALIDATION Abstract: Subtyping below the serovar level is essential for ...

Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.

PCR assay. Therefore, we here describe our observations made during the optimisation of a 20-plex MOL- PCR assay for subtyping of Salmonella Typhimurium with the aim to provide a possible workflow as ...

Evaluation of whole genome sequencing for routine subtyping of Salmonella Typhimurium for surveillance and outbreak investigation

Phenotype resistance routine S Salmonella Salmonella enterica Salmonella typhimurium Shigella situation Species study Subtyping Surveillance Technique technology whole genome whole genome sequencing Abstract: ...

MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.

demonstrates that MLVA improves public health surveillance of S. Typhimurium. However, the 5-loci MLVA should be complemented with other subtyping methods for investigation of possible outbreaks with frequent ...

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