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Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

purposes, both assays were run in parallel in a triplex one-step RT-qPCR protocol with beta-actin as an internal control and synthetic RNA as an external control. We obtained low limits of detection and high ...

Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens.

Diseases Specific Pathogen-Free Organisms Vaccination Vaccines, Synthetic Viral Structural Proteins Viral Vaccines Abstract: Infectious bursal disease (IBD) is an avian viral disease that causes severe ...

Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli.

Synthetic Vaccines, Virus-Like Particle Veterinary Medicine Viral Matrix Proteins Abstract: Virus-like particles (VLP s) are promising molecular structures for the design and construction of novel vaccines, ...

Protection Afforded by a Recombinant Turkey Herpesvirus-H5 Vaccine Against the 2014 European Highly Pathogenic H5N8 Avian Influenza Strain.

Birds Influenza Vaccines Vaccination Vaccines, Synthetic Abstract: A highly pathogenic avian influenza (HPAI) H5N8 (clade 2.3.4.4) virus, circulating in Asia (South Korea, Japan, and southern China) since ...

The combination of attenuated Newcastle disease (ND) vaccine with rHVT-ND vaccine at 1 day old is more protective against ND virus challenge than when combined with inactivated ND vaccine.

Vaccines, Synthetic Viral Fusion Proteins Viral Vaccines Abstract: The recurrent outbreaks of fatal Newcastle disease (ND) in commercial poultry flocks throughout the world indicate that routine vaccinations ...

Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.

C57BL Polyketide Synthases Vaccination Vaccines, DNA Vaccines, Synthetic Abstract: There is no effective vaccine against Buruli ulcer. In experimental footpad infection of C57BL /6 mice with M. ulcerans, ...

Detection of total and PRRSV-specific antibodies in oral fluids collected with different rope types from PRRSV-vaccinated and experimentally infected pigs.

fluid collected from PRRSV-vaccinated and infected pigs. RESULTS: An initial experiment showed that IgA is the predominant antibody isotype in porcine saliva. Moreover, it was found that synthetic ropes ...

Efficacy of marker vaccine candidate CP7_E2alf against challenge with classical swine fever virus isolates of different genotypes.

Vaccination Vaccines, Marker Vaccines, Synthetic Viral Vaccines Abstract: Classical swine fever (CSF) is among the most important viral disease of domestic and feral pigs and has a serious impact on animal ...

Genotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytes.

synthetic amorphous silica (SAS) and multiwalled carbon nanotubes (MWCNT s), in human lymphocytes. The morphology and size of those NM s were characterized by transmission electron microscopy, while the ...

Quantitative characterization of agglomerates and aggregates of pyrogenic and precipitated amorphous silica nanomaterials by transmission electron microscopy.

characterize the aggregates and agglomerates of precipitated and pyrogenic synthetic amorphous silicon dioxide (SAS), or silica, NM. RESULTS: Bright field (BF) TEM combined with systematic random imaging and ...

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