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Assessing the endocrine activity of PAHs using the DRE-, ERE- and PPARγ CALUX bioassays
expression) bioassays were used to determine the endocrine activity: 1)The PPAR y CALUX ® system of BioDetection systems (BDS, Amsterdam) uses U‐ 2OS cells (human osteoblast) that are stably transfected with ...
Immunogenicity and protective efficacy of tuberculosis DNA vaccines combining mycolyl-transferase Ag85A and phosphate transport receptor PstS-3.
Immunity, Cellular mice Mice, Inbred BALB C Mice, Inbred C57BL Mycobacterium tuberculosis Plasmids Recombinant Fusion Proteins Th1 Cells Transfection Tuberculosis Tuberculosis Vaccines Vaccines, DNA Abstract: ...
Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny.
nucleotide sequencing of cloned cDNA corresponding to isolate 256 revealed several mutations seven of which were attributed to the cloning procedure. The infectious transcript was transfected into permissive ...
Development of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning35
bacteria and able to regenerate virions when transfected into permissive cells. Reconstituted virus replicated comparably to wild-type parental virus and the loxP-flanked BAC cassette was excised by growing ...
Pharmacology of stomoxytachykinin receptor depends on second messenger system4403
were stably transfected with the cloned STKR cDNA. Pronounced differences in pharmacology were observed between agonist-induced calcium and cyclic AMP responses. The results indicate that the ...
Isoforms of cyclic AMP response element binding proteins in Drosophila S2 cells4408
transfected with a CRE-containing reporter gene construct. In combination with recent data from the literature, our results indicate that the regulation of CRE-dependent gene expression shows some important ...
Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells4409
pharmacology physiology plant Print Promoter Regions,Genetic protein Proteins Proteomics Receptor receptors Receptors,Steroid response SB- IM specific study System Systems Transcription Factors Transfection ...
Pharmacological characterization of STKR, an insect G protein-coupled receptor for tachykinin-like peptides480
receptors. Insect tachykinin-related peptides, also referred to as “insectatachykinins,” produce dose-dependent calcium responses in Drosophila melanogaster Schneider 2 cells, which are stably transfected with ...
Phenolamine-dependent adenylyl cyclase activation in Drosophila Schneider 2 cells481
to avoid cross-reactions, it is extremely important to know which endogenous receptors are already present in the non-transfected S2 cells. Therefore, we analyzed cellular levels of cyclic AMP and ...
Characterization of a receptor for insect tachykinin-like peptide agonists by functional expression in a stable Drosophila Schneider 2 cell line483
protein Proteins Receptor receptors Receptors,Invertebrate Peptide Receptors,Tachykinin Research Research Support response Responses SB- IM Tachykinins toxin Transfection Abstract: STKR is an insect ...