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Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

sequencing PCR-based enrichment single nucleotide variations Transgenic Rice Abstract: Similar to genetically modified organisms (GMO s) produced by classical genetic engineering, gene-edited (GE) organisms ...

Strategies to detect unauthorized GMO in the food and feed chain (PhD thesis)

GMO detection system using high-tech approaches. First, as a study case, an overview of genetically modified (GM) rice, developed around the world was carried out to collect information related inter ...

Strategies to detect unauthorized genetically modified organisms in food and feed chain

unauthorized GMO, this PhD aims to improve and strengthen the existing GMO detection system using high-tech approaches. First, as a study case, an overview of genetically modified (GM) rice, developed around the ...

Biotech Rice: Current developments and future detection challenges in food and feed chain

& Technology, Volume 52, Number 79 (2016) Keywords: Biotech crop detection Transgenic Rice Abstract: Background To improve agricultural practices and the food/feed security, plant breeding techniques ...

Integrated DNA walking system to characterize a broad spectrum of GMOs in food/feed matrices.

targeting the t35S pCAMBIA element. Methods Food/feed matrices containing transgenic crops (Bt rice or MON863 maize) were analysed using the integrated DNA walking system. Results First, the newly developed ...

Validation of a sensitive DNA walking strategy to characterise unauthorised GMOs using model food matrices mimicking common rice products

Food Chemistry, Volume 173 (2015) Keywords: detection DNA walking Rice food SENSITIVITY Transgene flanking region Unauthorised GMO Abstract: To identify unauthorised GMO s in food and feed matrices, an ...

An innovative and integrated approach based on DNA walking to identify unauthorised GMOs

147 (2014) Keywords: DNA walking GM rice GMO detection Integrated approach pCAMBIA vector Quantitative real-time PCR Transgene flanking region Unauthorised GMO Abstract: In the coming years, the ...

An innovative integrated approach based on DNA walking to identify unauthorized GMOs

family vector, frequently present in transgenic plants. The presented integrated approach is performed in two main successive steps on Bt rice grains. First, the potential presence of unauthorized GMO s is ...

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