Search results - 32 results

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three ...

Phylogeography of Human and Animal Coxiella burnetii Strains: Genetic Fingerprinting of Q Fever in Belgium

Variable-number Tandem Repeat Analysis (MLVA) (n=146), Single-Nucleotide Polymorphism (SNP) (n=92) and Whole Genome Sequencing (WGS) (n=4) methods were used to characterize samples/strains during 2009-2019. Three ...

A bioinformatics WGS workflow for clinical complex isolate analysis, validated using a reference collection extensively characterized with conventional methods and approaches.

Keersmaecker; Nancy Roosens; Marchal, Kathleen; Vanessa Mathys; Vanneste, Kevin Source: J Clin Microbiol (2021) Abstract: The use of whole genome sequencing (WGS) for routine typing of bacterial isolates has ...

Whole-genome sequencing for TB source investigations: principles of ethical precision public health.

González-Candelas, F; Forier, A; Juengst, E Source: Int J Tuberc Lung Dis, Volume 25, Issue 3 (2021) Abstract: Whole-genome sequencing (WGS) of allows rapid, accurate inferences about the sources, location and timing ...

Deepening of In Silico Evaluation of SARS-CoV-2 Detection RT-qPCR Assays in the Context of New Variants

Source: Genes, Volume 12, Issue 4 (2021) Keywords: SARS-CoV-2; COVID-19; variants; detection; RT-qPCR; in silico specificity evaluation; bioinformatics tool; WGS data; mismatches; primers and probes ...

Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.

Source: Eur Respir J (2020) Abstract: Conventional molecular tests for detecting complex (MTBC) drug resistance on clinical samples cover a limited set of mutations. Whole genome sequencing (WGS) typically ...

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relevant information for strain ...

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.

WGS whole genome sequencing Abstract: Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, ...

Use of a Whole Genome Sequencing-based approach for Mycobacterium tuberculosis surveillance in Europe in 2017-2019: an ECDC pilot study.

Stefan; van Soolingen, Dick; van der Werf, Marieke J; Cirillo, Daniela Maria Source: Eur Respir J (2020) Abstract: Whole genome sequencing (WGS) can be used for molecular typing and characterisation of ...

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