Search results - 7 results
Transforming Shiga toxin-producing surveillance through whole genome sequencing in food safety practices.
Abstract: INTRODUCTION: Shiga toxin-producing (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
Kathleen Marchal; Nancy Roosens; Sigrid C.J. De Keersmaecker; Kevin Vanneste Source: Microbial Genomics, Volume 7, Issue 3 (2021) Abstract: Whole genome sequencing (WGS) enables complete characterization of ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
demonstrating a data analysis approach for detailed characterization and phylogenetic placement of multiple bacterial strains of one species from shotgun metagenomic WGS data of an enriched food sample. Health ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
chain reactions (qPCRs) are used in routine. In recent years, the use of whole genome sequencing (WGS) of bacterial isolates has proven its value to collect relevant information for strain ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Foods, Volume 9, Issue 8 (2020) Keywords: Food Safety foodborne outbreak investigation Shiga toxin-producing Escherichia coli STEC Surveillance WGS ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
C.J. De Keersmaecker Source: Sci Rep, Volume 10, Issue 1 (2020) Keywords: NGS pathogenic E. coli STEC WGS Abstract: Whole genome sequencing (WGS) has proven to be the ultimate tool for bacterial isolate ...