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amplification

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Targeted Whole Genome Sequencing of the Capripoxvirus Genome from Clinical Tissue Samples and Lyophilized Vaccine Batches.

of tiled amplicons. The resulting amplicons can be sequenced on all currently available high-throughput sequencing platforms. As an example, we have included a detailed protocol for performing nanopore ...

Genomic monitoring of SARS-CoV-2 variants using sentinel SARI hospital surveillance.

patients fulfilling the Belgian SARI definition between January 2020 and December 2022 were sequenced using the ARTIC Network amplicon tiling approach on a MinION platform. Weekly variant of concern (VOC) ...

A Summer of Cyanobacterial Blooms in Belgian Waterbodies: Microcystin Quantification and Molecular Characterizations

sequencing. The cyanobacterial diversity for two water samples was characterized with amplicon sequencing. Microcystins were detected above limit of quantification (LOQ) in 68 water samples, and the World ...

Strategy and Performance Evaluation of Low-Frequency Variant Calling for SARS-CoV-2 Using Targeted Deep Illumina Sequencing

of interest into raw wild-type sequencing data, or by mixing wild-type and mutant raw sequencing data, to construct mixed samples subjected to WGS using a tiling amplicon-based targeted metagenomics ...

Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.

Publication Type: Peer reviewed scientific article Authors: Agathe Jouet; Cyril Gaudin; Nelly Badalato; Allix-Béguec, Caroline; Stéphanie Duthoy; Alice Ferré; Maren Diels; Yannick Laurent; Sandy Contreras; Silke Feuerriegel; Stefan Niemann; Emmanuel André ...

Development of a genoserotyping system for the identification of Salmonella serotypes

a capturing of the created amplicons by specific oligonucleotides coated on color-coded microspheres, which are themselves detected by a device through a fluorescence reaction (Luminex technology). ...

Discrimination of three genetically close Aspergillus species by using high resolution melting analysis applied to indoor air as case study.

the highly accurate determination of the difference in melting temperature of the obtained amplicon. In this study, we provide a proof-of-concept for this approach, using a dye adapted version of our ...

Four new SYBR®Green qPCR screening methods for the detection of Roundup Ready(r), LibertyLink(r), and CryIAb traits in genetically modified products362

traits as present in genetically modified organisms (GMO) were developed. Their specificity, sensitivity, and PCR method efficiency were determined. All methods are specific and generate amplicons of 108, ...

Epidemiological study of bovine norovirus infection by RT-PCR and a VLP-based antibody ELISA.

diarrhoeic calves screened by RT- PCR. Nucleotide sequencing performed on the polymerase region classified all the norovirus amplicons in the bovine norovirus genotype 2. Rather surprisingly, some rotavirus ...

Arcobacter cibarius sp. nov., isolated from broiler carcasses.

PCR tests but that yielded an amplicon in an Arcobacter genus-specific PCR test were isolated from 13 unrelated broiler carcasses. Numerical analysis of the profiles obtained by SDS- PAGE of whole-cell ...

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