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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

Ultimately, a validation of the method is still necessary in order to obtain a precise limit of detection based on the analysis of a large dataset of samples. Moreover, other technologies can still be ...

Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS

extracted with methanol 80%, followed by an SPE clean-up step before LC- MS / MS analysis. The LOQ was fixed at 1 µg/L for the nine cyanotoxins and 0.05 µg/L for AFM1. Recoveries were measured between 82.67% ...

The contribution of cacao consumption to the bioaccessible dietary cadmium exposure in the Belgian population

the Belgian population, in combination with ICP- MS analysis of a large subset of cacao-containing products (N=349). Both the average chocolate consumption (28 g day-1) and the relative contribution of ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

propose a targeted high-throughput sequencing approach, including (i) a prior PCR-based enrichment step to amplify regions of interest, (ii) a sequencing step, and (iii) a data analysis methodology to ...

Aluminium intake through the consumption of selected baby foods and risk characterization in a population of Brazilian infants aged 0 to 36 months

Antônio Morgano; Arisseto-Bragotto, Adriana Pavesi Source: Journal of Food Composition and Analysis, Volume 115 (2023) Keywords: Aluminium Food Safety Risk Assessment Abstract: Aluminium (Al) dietary intake ...

Report on an initial assessment of long-read sequencing for metagenome (community and AMR) analysis (defined community, spiked and real simple and complex matrices) and comparison to current short-read standard

Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Sigrid C.J. De Keersmaecker; Mathieu Gand; Indre Navickaite; Josephine Gruetzke; Lee-Julia Bartsch; Saria Otani; Giuliano Garofolo; Marco Di Domenico; Valeria Michel ...

Optimised long-read sequence bioinformatics tool for the analysis of the resistome and microbial communities

Publication Type: Sci. report, recommendat°, guidance doc., directive, monograph Authors: Manal AbuOun; Saria Otani; Indre Navickaite; Mathieu Gand; Bram Bloemen; Kevin Vanneste; Valeria Michelacci; Bosco Rodríguez Matamoros; Nancy Roosens; Sigrid C.J. De ...

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