Search results - 169 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
Staphylococcus aureus) compared to shotgun metagenomics without adaptive sampling on DNA of mashed potatoes spiked with DNA of S. aureus at a level of 0.5%. The strain was previously associated with a foodborne ...
Cost and greenhouse gas emissions of current, healthy, flexitarian and vegan diets in Aotearoa (New Zealand)
generally at a higher cost to households. The results reflect trade-offs, with the larger constraints placed on diets, the greater cost and factors such as nutritional adequacy, variety, cost and ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
contaminant present in the sample at once, possibly also at the SNP level. At the time this study started, shotgun metagenomics for the study of food contaminants was in its infancy. Moreover, strain-level ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. New methods released on the market, and protocols developed at the research ...
Simultaneous Detection and Quantification of Aflatoxin M1, Eight Microcystin Congeners and Nodularin in Dairy Milk by LC-MS/MS
extracted with methanol 80%, followed by an SPE clean-up step before LC- MS / MS analysis. The LOQ was fixed at 1 µg/L for the nine cyanotoxins and 0.05 µg/L for AFM1. Recoveries were measured between 82.67% ...
Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms
enforcement laboratories is required by the competent authorities to guarantee food/feed safety and traceability (2003/1829/ EC; 2003/1830/ EC). However, their detection is potentially challenging at both the ...
Regulatory safety assessment of nanoparticles for the food chain in Europe
that may retain properties at the nanoscale. Key parameters and threshold values for establishing the presence of small particles, the techniques and methods for characterisation in complex matrices, as ...
Aluminium intake through the consumption of selected baby foods and risk characterization in a population of Brazilian infants aged 0 to 36 months
for the total population, calculated using mean Al concentrations, were estimated to be 0.184 mg/kg body weight (bw) per week, whilst at 95th percentile, the obtained value was 0.474 mg/kg bw per week. ...
Pesticides, trace elements and pharmaceuticals in tea samples available in Belgian retail shops and the risk associated upon acute and chronic exposure
performed a risk assessment for these samples. No chemical adulteration could be detected in dry tea material. More than 38% of the dry leaves samples contained at least one pesticide exceeding the maximal ...
Methodological choices in brucellosis burden of disease assessments: A systematic review
estimates at the global level, the rest were national or subnational assessments. Data regarding different methodological choices were extracted, including detailed assessments of the adopted disease models. ...