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The GEN-ERA toolbox: unified and reproducible workflows for research in microbial genomics
need to implement new standards in species delineation, including whole-genome sequencing and phylogenomics. In this context, the genomic needs of the Belgian Coordinated Collections of Microorganisms ...
Retrospective surveillance of viable Bacillus cereus group contaminations in commercial food and feed vitamin B2 products sold on the Belgian market using whole-genome sequencing
Kevin Vanneste Source: Frontiers in Microbiology, Volume 14 (2023) Keywords: Bacillus cereus Food Additives riboflavin Surveillance whole-genome sequencing Abstract: Bacillus cereus is a spore-forming ...
Outbreak of Corynebacterium diphtheriae among asylum seekers in Belgium in 2022: operational challenges and lessons learnt.
2022, 25 cases of toxigenic infection were confirmed among asylum seekers, mostly among young males from Afghanistan. Multi-locus sequence typing showed that most isolates belonged to sequence types 574 ...
BSFM 2023- L. monocytogenes in Plant-Based Foods: insights from of a recent foodborne outbreak linked to vegan alternative to cheese
following ISO 11290 – 1 and – 2. A human strain was collected at the NRC Listeria. All isolated strains underwent serotyping for further characterisation at the NRC Listeria, and Whole Genome Sequencing (WGS) ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
have always been analyzed and characterized after isolation. Since the advent of second generation sequencing, it has been possible to characterize the complete genome of these isolates to the SNP level. ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
improves the reconstruction of genomes and helps to resolve complex genomic contexts, especially in the case of metagenome analysis. To take the best advantage of long-read sequencing, DNA extraction methods ...
ESCAIDE 2022 Oral presentation: International outbreak of Salmonella Typhimurium linked to a chocolate factory in 2022: Belgian findings
were analysed for Salmonella spp. using real-time PCR. Whole genome sequence (WGS) analysis of isolates of probable cases and positive food samples is still ongoing. Results We identified 62 probable ...
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins
Issue 8 (2022) Keywords: Food Safety microcystin Microcystis sequencing Tandem Mass Spectrometry; BGAS ultra-high performance liquid chromatography Abstract: Food supplements are gaining popularity ...
LC-MS/MS Validation and Quantification of Cyanotoxins in Algal Food Supplements from the Belgium Market and Their Molecular Origins.
worldwide. However, harmful natural compounds can contaminate these products. In the case of algae-based products, the presence of toxin-producing cyanobacteria may cause health risks. However, data about the ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
defined as the study of all genetic material within a sample, using sequencing technologies. Shotgun metagenomics is capable of detecting a wide range of microorganisms in a sample, as well as their ...