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Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.
Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using ...
Implementation of MALDI-TOF Mass Spectrometry to Identify Fungi From the Indoor Environment as an Added Value to the Classical Morphology-Based Identification Tool
collaboration with Brussels Environment RCIB / CRIPI and identified by both microscopy and MALDI- TOF MS in Sciensano’s Indoor Mycology laboratory. Spectra obtained via MALDI- TOF MS were compared with data ...
Development of a Droplet Digital PCR to Monitor SARS-CoV-2 Omicron Variant BA.2 in Wastewater Samples
wild-type probe of the T19I assay was measured and the proportion of genomes with the T19I mutation, characteristic of the BA.2 mutant, compared to the entire SARS-CoV-2 population was calculated. The ...
Hemorrhagic bowel syndrome in dairy cattle: Gross, histological, and microbiological characterization.
erosions or lacerations measuring 4 to 45 mm. In 77% (37/48) of these mucosal lesions, microscopic splitting of the comparable to the hematoma was present. These findings suggest the intramucosal hematoma to ...
Papilloplex HR-HPV test has non-inferior clinical performance for detection of human papillomavirus infection: assessment using the VALGENT framework.
The Papilloplex HR- HPV test has non-inferior sensitivity for detection of cervical precancer (p=0.0001 for ≥ CIN2 and p=0.0005 for ≥ CIN3) and non-inferior specificity, compared with GP5 +/6+ EIA ...
Assessment of the clinical and analytical performance of the Aptima SARS-CoV-2 assay using the VALCOR protocol.
a standard comparator assay. Diagnostic test parameters such as sensitivity, specificity and overall per cent agreement were calculated for the clinical performance of Aptima SARS-CoV-2 assay. RESULTS: A total ...
Homologous and Heterologous Prime-Boost Vaccination: Impact on Clinical Severity of SARS-CoV-2 Omicron Infection among Hospitalized COVID-19 Patients in Belgium
estimated risk for severe COVID-19 and in-hospital mortality was significantly lower with an mRNA booster vaccination as compared to only a primary vaccination schedule (RR = 0.59 [0.33; 0.85] and RR = 0.47 ...
HPV-based Cervical Cancer Screening on Self-samples in the Netherlands: Challenges to Reach Women and Test Performance Questions.
self-sampling (16% in 2020). HPV positivity and detection rate of precancer (CIN3 +) were lower in the self-sampling compared with the clinician-sampling group (adjusted OR s of 0.65 and 0.86, respectively). ...
Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence.
relative sensitivities of 23% and 20%, respectively, compared to the PRNT results. The relative specificities were 88% and 84% due to cross reactions with USUTU virus-specific antibodies. The minimal TBEV ...
Clinical Evolution and Disease Burden in Belgian Cystic Fibrosis Patients: effect of Newborn Screening?
Issue 4, Number 297 (2023) Abstract: Objectives: We compared the clinical evolution and disease burden of 3 groups of Belgian children with cystic fibrosis (CF) depending on the mode of diagnosis: ...