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Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.
Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using ...
Bigger and Better? Representativeness of the Influenza A Surveillance Using One Consolidated Clinical Microbiology Laboratory Data Set as Compared to the Belgian Sentinel Network of Laboratories.
Publication Type: Peer reviewed scientific article Authors: Van den Wijngaert, Sigi; Nathalie Bossuyt; Bridget Ferns; Busson, Laurent; Gabriela Serrano; Wautier, Magali; Isabelle Thomas; Matthew Byott; Yves Dupont; Eleni Nastouli; Hallin, Marie; Zisis Koz ...
Comparative Profiling of Ubiquitin Proteasome System Interplay with Influenza A Virus PB2 Polymerase Protein Recapitulating Virus Evolution in Humans
comparative interactomics of multiple strains is an effective strategy to highlight correlations between host proteome hijacking and biological or pathogenic traits. Here, we developed an interactomic pipeline ...
Temperature sensitivity on growth and/or replication of H1N1, H1N2 and H3N2 influenza A viruses isolated from pigs and birds in mammalian cells.
viruses belonging to different lineages isolated from birds or pigs prior to 2005 was tested on MDCK or NPT r cell lines in the presence or absence of exogenous trypsin. Virus multiplication was compared at ...
Non coding extremities of the seven influenza virus type C vRNA segments: effect on transcription and replication by the type C and type A polymerase complexes.
CAT reporter gene were comparable in the presence of either type C or type A polymerase complexes except for the NS and PB2-like vRNAs. For the NS-like vRNA, the transcription/replication level was ...
Influenza epidemic surveillance and prediction based on electronic health record data from an out-of-hours general practitioner cooperative: model development and validation on 2003-2015 data.
METHOD: Validity of the OOH GPC data was assessed by comparing OOH GPC ILI data with WIV- ISP ILI data for the period 2003-2012 and using Pearson’s correlation. The best fitting prediction model based on ...