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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

Staphylococcus aureus) compared to shotgun metagenomics without adaptive sampling on DNA of mashed potatoes spiked with DNA of S. aureus at a level of 0.5%. The strain was previously associated with a foodborne ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

microbiology. Compared to short read sequencing (Illumina), this technology allows to unambiguously detect and scaffold microbial genes to their host chromosomes, even for complex metagenomics samples, allowing ...

Biological activity conferred by endocrine disrupting chemicals in hospital effluent and river water from the Brussels Region, Belgium

assess endocrine activity by comparing  the  mixture  or  cocktail  effect  originating  from  the  sample  extract.  Added  value  is  that  this technique picks up effects from compounds that are  not ...

A proteomics approach for the quantification of Staphylococcus aureus enterotoxins a and b in several matrices using uplc-msms analysis.

validating this method according to ISO 2002/657/ EC. When comparing the MS spectra peaks found for the IS and the endogenous peptides we were able to identify and quantify the presence of SEA and/or SEB in ...

Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology

and cost of experimentation.We have compared two commercial software packages for the development and in silico simulation of a ligation dependent amplification (LDA) assay and a direct hybridization ...

An Alternative Method Validation Strategy for the European Decision 2002/657/EC

performance characteristic is not increased since the degrees of freedom are equal or higher compared to the conventional validation plan. Only 24 samples (27 samples for a balanced design) are needed with this ...

Misreporting of energy intake in the belgian food consumption surveys (2004-2014)

compared with FCS2004 for participants aged 15-64. Results Overall in 2014, 24.3% of participants underreported, 75.2% were plausible reporter and 0.5% overreported. Females underreported more often than ...

Accumulation-depuration potential and natural occurrence of Microcystin-LR toxin in basil

kg-1 and 143.80 µg kg-1, respectively. The basil roots accumulated more toxin compared to the leaves. Depuration was observed for all treatment conditions in both roots and leaves. At least six ...

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