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A new mouse model of lung allergy induced by the spores of Alternaria alternata and Cladosporium herbarum molds.
alternata and C. herbarum spores. Bronchoalveolar lavages (BAL s) from these mice contained numerous macrophages, neutrophils, eosinophils and lymphocytes whereas neutrophils were the predominant BAL ...
Streptomycin as a selective agent to facilitate recovery and isolation of introduced and indigenous Sphingomonas from environmental samples.
culturable Sphingomonas from soils. All 29 previously described bacterial strains belonging to 17 different Sphingomonas species were able to grow on mineral media containing 200 microg ml(-1) streptomycin, ...
Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe.
prepared, lyophilised and distributed by 1 laboratory to 12 reference laboratories in Europe. The serum sets contained the three European bovine herpesvirus 1 (BHV1) reference serum samples (EU1, EU2 and ...
Conditional expression of type I interferon-induced bovine Mx1 GTPase in a stable transgenic vero cell line interferes with replication of vesicular stomatitis virus.
We validated a double transgenic Vero cell clone in which the bovine Mx1 reference allele is placed under control of the human cytomegalovirus (CMV) enhancer-promoter sequence containing elements from ...
Isoforms of cyclic AMP response element binding proteins in Drosophila S2 cells4408
transfected with a CRE-containing reporter gene construct. In combination with recent data from the literature, our results indicate that the regulation of CRE-dependent gene expression shows some important ...
Substitution of conserved glycine residue by alanine in natural and synthetic neuropeptide ligands causes partial agonism at the stomoxytachykinin receptor4407
peptides, such as stomoxytachykinin (Stc- TK), contain an Ala-residue instead of the highly conserved Gly-residue that is present in most other members of this peptide family. Stc- TK is a potent, partial ...
Biosafety risk assessment of the Severe Acute Respiratory Syndrome (SARS) coronavirus and containment measures for the diagnostic and research laboratories
assessment at Belgium biosafety Case containment Control Coronavirus Countries disease environment Europe Guidelines health Human INFECTION International investigation IS Laboratories LEVEL levels measure ...
Analysis of Ah receptor pathway activation by brominated flame retardants
2,2’,4,4’,5,5’-hexabromobiphenyl. Some technical mixtures of brominated flame-retardants contain brominated biphenyls, dioxins or dibenzofurans as contaminants. When tested in the CALUX assay these compounds were found to be ...
Functional and comparative analysis of two distinct ecdysteroid-responsive gene expression constructs in Drosophila S2 cells4409
elements. These minimal promoters, either containing a TATA-box or a downstream promoter element, drove the expression of a luciferase reporter gene. Potent induction capacities were observed with the insect ...
Glucocorticoid-inducible gene expression vectors for use in Drosophila melanogaster Schneider 2 cells4410
to one of three distinct types of Drosophila gene promoters: (1) a TATA-box containing, (2) a TATA-less and (3) a bidirectional core sequence. These were fused to a luciferase reporter gene. The ...