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Attributing Ethiopian animal health losses to high-level causes using expert elicitation

high-level causes (infectious, non-infectious, and external). Separate in-person workshops were conducted with eight cattle, nine small ruminant, and eight chicken experts. Following the ...

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

purposes, both assays were run in parallel in a triplex one-step RT-qPCR protocol with beta-actin as an internal control and synthetic RNA as an external control. We obtained low limits of detection and high ...

Validation of a particle tracking analysis method for the size determination of nano- and microparticles.

p.271 (2017) Abstract: Particle tracking analysis (PTA) is an emerging technique suitable for size analysis of particles with external dimensions in the nano- and sub-micrometre scale range. Only limited ...

Identification of risk factors for Campylobacter contamination levels on broiler carcasses during the slaughter process.

external carcass contamination and the colonization level of caeca) and the duration of transport and holding time that can be linked with feed withdrawal period. In addition, technical aspects of the ...

A survey on biosecurity and management practices in selected Belgian cattle farms.

enhancement of preventive measures considering external and internal biosecurity was still possible for each farm, as none of the farms obtained an overall high biosecurity level. Three groups of cattle farms ...

Vers un monitoring des facteurs de risque d'émergence des maladies animales?

Santé animale Diergezondheid Service:  Pathogènes alimentaires Voedselpathogenen Foodborne pathogens Strategy external positioning Strategie en externe positionering Stratégie et positionnement externe ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

become the method of choice for routine viral diagnosis. As false-negative test results can have serious implications; an internal/external control system should be incorporated in each analysis to detect ...

A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.

results, a triplex RT-qPCR was used which detects the target viral RNA, an internal and an external control. The methods to determine the validation parameters such as linearity, efficiency, analytical ...

Development of an M2e-specific enzyme-linked immunosorbent assay for differentiating infected from vaccinated animals.

discriminating between vaccinated and virus-infected poultry. We studied M2 e, the highly conserved external domain of the influenza A M2 protein, as a potential differential diagnostic marker for influenza virus ...

Foot-and-mouth disease virus: a first inter-laboratory comparison trial to evaluate virus isolation and RT-PCR detection methods.

from high in one laboratory, moderate in two and low in two others. This prototype panel of samples would appear suitable for external quality assurance of these tests but would benefit from the ...

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