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Targeted Chromosomal Barcoding Establishes Direct Genotype-Phenotype Associations for Antibiotic Resistance in Mycobacterium abscessus.

isolate. The allelic exchange substrates used in recombineering carried either the sole D29A mutation or a genetic barcode of silent mutations in codons flanking the D29A mutation. After selection of ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants.

Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using ...

Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage.

exposure limits could result in adverse human health effects. In the present study, the effects of long-term MF exposure on three different study endpoints (cell viability, genetic damage, and sensitivity to ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

based on the sequencing of all genetic material of the sample without isolation has become available some years ago, e.g. shotgun metagenomics. It allows to get a screenshot of every microbiological ...

Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products

Recently, a genetically modified microorganism (GMM) detection strategy using real-time PCR technology was developed to control fermentation products commercialized in the food and feed chain, allowing ...

Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage

Publication Type: Peer reviewed scientific article Authors: Nguyen, Ha; Segers, Seppe; M. Ledent *; Anthonissen, Roel; Luc Verschaeve *; Hinsenkamp, Maurice; J.-F. Collard; Feipel, Veronique; Mertens, Birgit Source: Heliyon, Volume 9, Issue 3 (2023) Healt ...

Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants

Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde *; François Dufrasne *; Steven Van Gucht *; Xavier Saelens; Nancy Roosens * Source: Current Issues in Molecular Biology, Volume 45, Issue 3 (2023) Health Topics:  Health and ...

Effects of long-term exposure to 50 Hz magnetic fields on cell viability, genetic damage, and sensitivity to mutagen-induced damage

Publication Type: Peer reviewed scientific article Authors: Nguyen, Ha; Segers, Seppe; M. Ledent *; Anthonissen, Roel; Luc Verschaeve; Hinsenkamp, Maurice; Collard, Jean-Francois; Feipel, Veronique; Mertens, Birgit Source: Heliyon, Volume 9, Issue 3 (2023 ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...

Metagenomic Characterization of Multiple Genetically Modified Bacillus Contaminations in Commercial Microbial Fermentation Products

12, Issue 12 (2022) Keywords: Bacillus food enzyme genetically modified microorganisms (GMM) hybrid genome assembly metagenomic shotgun sequencing Abstract: Genetically modified microorganisms (GMM) are ...

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