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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...

Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety

based on the sequencing of all genetic material of the sample without isolation has become available some years ago, e.g. shotgun metagenomics. It allows to get a screenshot of every microbiological ...

Targeted High-Throughput Sequencing Enables the Detection of Single Nucleotide Variations in CRISPR/Cas9 Gene-Edited Organisms

Sigrid C.J. De Keersmaecker; Kevin Vanneste; Nancy Roosens Source: Foods, Volume 12, Issue 3 (2023) Keywords: CRISPR/Cas9 detection food and feed chain Genetically modified organism high-throughput ...

Development of a taxon-specific real-time PCR method targeting the Bacillus subtilis group to strengthen the control of genetically modified bacteria in fermentation products

flavorings Bacillus subtilis group Enzymes food and feed safety concerns real-time PCR detection Unauthorized genetically modified microorganisms Abstract: Most of the bacteria that are used to produce ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

defined as the study of all genetic material within a sample, using sequencing technologies. Shotgun metagenomics is capable of detecting a wide range of microorganisms in a sample, as well as their ...

Listeria monocytogenes isolates from Cornu aspersum snails: Whole genome-based characterization and host-pathogen interactions in a snail infection model.

expression led only to an increase of necrotic non-apoptotic cells. Overall, this study provides significant insights into the genetic diversity of L. monocytogenes from snails, the genomic features of them ...

Development of a real-time PCR marker targeting a new unauthorized genetically modified microorganism producing protease identified by DNA walking

Microbiology, Volume 354 (2021) Keywords: Antimicrobial resistance genes DNA walking anchored on pUB110 food and feed safety Genetically modified bacteria Protease real-time PCR detection Abstract: A PCR-based ...

Adjunct Culture of Non-Starter Lactic Acid Bacteria for the Production of Provola Dei Nebrodi PDO Cheese: In Vitro Screening and Pilot-Scale Cheese-Making.

were genetically identified by Rep- PCR genomic fingerprinting, using the (GTG)-primer, and by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI- TOF MS). Twenty-seven ...

A shotgun metagenomics approach to detect and characterize unauthorized genetically modified microorganisms in microbial fermentation products

Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Food Chemistry: Molecular Sciences, Volume 2 (2021) Keywords: AMR genetically modified microorganism identification long and short read sequencing ...

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