Search results - 79 results
Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11.
RT-qPCR assay. In silico and in vitro analyses indicated that none of the assays cross-react with viruses which are symptomatically or genetically related to BTV and only detect the intended BTV serotypes. ...
Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 136839
1 (MNV-1) real-time PCR detection assay described recently was integrated successfully into the multiplex assay, making it possible to detect GI and GII NoVs and MNV-1 in one reaction tube with MNV-1 plasmid ...
Collaborative Study for the Validation of Alternative in vitro Potency Assays for Human Tetanus Immunoglobulin
vivo assay to be performed to determine the potency of human tetanus immunoglobulins during their development. Furthermore, it states that an in vitro method shall be validated for the batch potency ...
In vitro investigations related to the hypothesis that Lipoatrophia semicircularis finds its origin in electro-stimulation
comet assay electric current electro-sensitivity lipoatrophia semicircularis Macrophage Health Topics: Non-ionising radiation Rayonnements non-ionisants Niet-ioniserende straling Service: Contaminants ...
Novel highly potent mu-opioid receptor antagonist based on endomorphin-2 structure4392
[Dmt(1),Sar(2),d-2-Nal(4)]endomorphin-2 possessed very high affinity for the mu-opioid receptor (IC (50)=0.01+/-0.001 nM) and turned out to be a potent and extremely selective mu-opioid receptor antagonist, as judged by the in vitro ...
Mu-opioid receptor ligands lack receptor subtype selectivity in the aequorin luminescence-based calcium assay4393
characterize the binding selectivity of the mu-opioid receptor ligands, endomorphin-1, endomorphin-2, and DAMGO, in the in vitro functional assay, based on the changes in intracellular calcium levels. For the ...
A novel method to quantify in vivo transferrin glycation: applications in diabetes mellitus
result results serum stress study Transferrin Type Universities university VARIABILITY Abstract: BACKGROUND: In vitro glycation of transferrin leads to increased oxidative stress by impairing iron-binding ...
A novel method to quantify in vivo transferrin glycation: applications in diabetes mellitus101
parameters performance Print Research Research Support result results SB- IM serum stress study Transferrin Type Universities university VARIABILITY Abstract: BACKGROUND: In vitro glycation of transferrin ...
[D-1-Nal4]endomorphin-2 is a potent micro-opioid receptor antagonist in the aequorin luminescence-based calcium assay4398
endomorphin-2 in the in vivo hot-plate test in mice, was tested in the aequorin luminescence-based calcium assay to examine their micro-opioid antagonist potency in vitro. A recombinant mammalian cell line ...
Interference of dextran in biuret-type assays of serum proteins120
Supplementation survey Universities university university hospital Abstract: Dextran interference in biuret-type assays of total serum proteins was investigated in a Belgian National External Quality Assurance ...