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Intra-host variation structure of classical swine fever virus NS5B in relation to antiviral therapy.
antivirals, in respect to antiviral treatment. Using the non-unique nucleotide changes, a limited intra-host variation was found in the wild type virus with 2 silent and 2 non-synonymous sites. This number ...
Development and validation of qualitative SYBR®Green real-time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes.
of Listeria spp. The selected primers were designed to amplify short fragments (60 to 103 bp) in order to obtain optimal PCR efficiency (between 97 and 107 % efficiency). The limit of detection of the ...
Efficacy of chimeric Pestivirus vaccine candidates against classical swine fever: protection and DIVA characteristics.
with C-strain, CP7_E2alf and Flc11. Limited transmission occurred in the groups vaccinated with Flc9. All vaccine candidates would be suitable to stop on-going transmission of CSFV. For Flc11, no ...
A multifaceted study of Pseudomonas aeruginosa shutdown by virulent podovirus LUZ19
knowledge on genome content and diversity of bacterial viruses, insights in intracellular phage development and its impact on bacterial physiology are very limited. We present a multifaceted study combining ...
Lymph node targeting of BCG vaccines amplifies CD4 and CD8 T-cell responses and protection against Mycobacterium tuberculosis34032
provides limited protection against pulmonary tuberculosis and a risk of dissemination in immune-compromised vaccinees. For the development of new TB vaccines that stimulate strong T-cell responses a variety ...
Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology
IT limitation Limitations Luminex microorganism microorganisms Molecular molecular subtyping Multiplex assay Oligonucleotides outbreak outbreaks pathogen reducing regulation Sample SOFTWARE specificity ...
Determination and use of processing factors for pesticide residues after processing of fruit and vegetables: carrot, celery, spinach, melon
example the application time of pesticide or the choice of variety. These elements support the relevance of a reassessment of agricultural practices in order to limit further risks for the consumer. ...
A validated GC-MS method for the determination and quantification of residual solvents in counterfeit tablets and capsules.
(2012) Keywords: Calibration Capsules Counterfeit Drugs Drug Contamination Fraud Gas Chromatography-Mass Spectrometry Limit of Detection Reference Standards Reproducibility of Results Solvents Tablets ...
Scientific opinion on emerging and novel brominated flame retardants (BFRs) in food
very limited information on occurrence, exposure and toxicity, the CONTAM Panel could not perform a risk characterisation for any of the BFR s considered. Instead, an attempt was made to identify those ...
Determination of Bacillus cereus Emetic Toxin in Food Products by Means of LC-MS²
describes the validation,according to the Commission Decision 2002/657/ EC, of the LC- MS ² method, a tandem mass spectrometry technique, which guarantees lower detection limit and higherspecificity. The LC- ...