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Pyrogen testing of phage therapeutic products
endotoxins, components of the cell wall of gram-negative bacteria which are released upon bacterial lysis. In our routine phage quality control (QC) workflow, endotoxins are quantified using the Limulus ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for ...
Evolution and determinants of glycaemic control in children and adolescents with type 1 diabetes in Belgium: A 10-year period observational real-world study
Publication Type: Scientific poster, presentation or proceeding Authors: Suchsia Chao; An-Sofie Vanherwegen; Dominique Beckers; Kristina Casteels; Daniel Klink; Philippe Lysy; Robrecht De Schreye ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
drug) are combined to one numeric value which determines the drug susceptibility of the investigated strain. The assay was optimized for parameters as cell density, incubation time and lysis method. ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) and its monophasic variant S. 1, 4[5],12:i:- from DNA isolation through heat lysis up to data interpretation through a Gödel Prime ...
Zona pellucida gene mRNA expression in human oocytes is related to oocyte maturity, zona inner layer retardance and fertilization competence.
levels to fertilization capacity is indirect as oocyte gene expression analysis required lysis of the oocyte. WIDER IMPLICATIONS OF THE FINDINGS: Overall relations between PLM observations, mRNA expression ...
Interaction of Aspergillus fumigatus conidia with Acanthamoeba castellanii parallels macrophage-fungus interactions.
observed with light and transmission electron microscopy. These processes resulted in structural changes in A. castellanii, leading to amoebal permeabilization without cell lysis. In conclusion, A. ...
Assessment of the efficacy of benzalkonium chloride and sodium hypochlorite against Acanthamoeba polyphaga and Tetrahymena spp.
g/liter) conditions. Benzalkonium chloride caused cell lysis at concentrations above 50 mg/liter under clean and dirty conditions. A concentration of 50 mg of free chlorine per liter had a strong biocidal ...
Contained use of Bacteriophages: Risk assessment and biosafety recommendations2463
environment epub Evaluating Evolution factors Field gene gene therapy gene transfer general Genetic Genome genomic health Human human health implement Laboratories lysis management measure measures medical ...
Functional characterization of in vivo effector CD4(+) and CD8(+) T cell responses in acute Toxoplasmosis: an interplay of IFN-gamma and cytolytic T cells36873
Cytotoxicity,Immunologic Development disease Dna DNA vaccines electronic Female Functional im immune response Immunization immunology in vivo INFECTION Institute Interferon-gamma IS journal Laboratories lysis Macrophage ...