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Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
of the drug. RNA was extracted combining bead-beating in TRI zol and the Direct-Zol purification kit. After quality control, the extracts were fragmented, barcoded and pooled, cDNA libraries were ...
Global transcriptional analyses of Mycobacterium tuberculosis using old and new drugs
bead-beating in TRI zol and the Direct-Zol purification kit. After quality control, the extracts were fragmented, barcoded and pooled, cDNA libraries were constructed and sequenced using the HiSeq technology ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
is incubated in absence of the drug. Multiplex mRNA quantification is performed directly on crude cell lysates using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo ...