Search results - 607 results

The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.

a 3’ random part used to prime complementary DNA synthesis and a 5’ defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next generation sequencing to ...

In vitro and in vivo infectious potential of coxiella burnetii: a study on Belgian livestock isolates.

in the Netherlands during the 2007-2010 outbreaks. Infective doses were established with ethidium monoazide- PCR for the first time here applied to C. burnetii. This method allowed for the preparation ...

Intake and dietary sources of haem and non-haem iron among European adolescents and their association with iron status and different lifestyle and socio-economic factors.

sources and; (2) to evaluate the relation of iron intake and status in European adolescents. SUBJECTS / METHODS: Two non-consecutive 24-h recalls were completed by a computerised tool. The socio-demographic ...

MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.

demonstrates that MLVA improves public health surveillance of S. Typhimurium. However, the 5-loci MLVA should be complemented with other subtyping methods for investigation of possible outbreaks with frequent ...

Routinely-collected general practice data from the electronic patient record and general practitioner active electronic questioning method: A comparative study

practitioners GP healthcare Hypertension improve index INFORMATION IS IT journal Mass Mass index method methods Network networks observed Paper parameters past Patient patients Practice practitioner Practitioners ...

End-of-life communication: a retrospective survey of representative general practitioner networks in four countries

method methods Netherlands Network networks objectives OCCUPATIONAL Occupational Health older ON Patient patients practitioner prevalence PRIORITIES Priority public result results SOCIAL Spain study survey ...

End-of-life decisions: a cross-national study of treatment preference discussions and surrogate decision-maker appointments

Logistic-regression medical method methods Netherlands Network networks OCCUPATIONAL Occupational Health Participation Patient patients Planning prevalence public regression Research Research Support result results SB- ...

Importance of identification and typing of Brucellae from West African cattle: a review.

international classification methods with no time limit and no language restrictions. Studies reporting results of identification at genus level only were not considered for this review. This review revealed that ...

Clinical value of IS6110-based loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis complex in respiratory specimens

isolation & purification IT journal medical Medicine method methods microbiology Molecular Typing Mycobacterium Mycobacterium tuberculosis Nucleic Acid Amplification Techniques objectives ON p Patient ...

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