Search results - 149 results
Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.
a 100-fold decrease in sensitivity. Using a nested PCR approach, the detection limit could be lowered to at least 10 cells g(-1) of soil. The method was applied to assess Acinetobacter diversity in soil ...
Analytical performance of several classical swine fever laboratory diagnostic techniques on live animals for detection of infection.
Publication Type: Peer reviewed scientific article Authors: Dewulf, Jeroen; F. Koenen; Mintiens, Koen; Denis, Pierre; Ribbens, Stefaan; de Kruif, Aart Source: J Virol Methods, Volume 119, Issue 2, ...
Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein.
Immunosorbent Assay Humans polymerase chain reaction Prions Sensitivity and Specificity Abstract: Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of ...
Decision support tools for clinical diagnosis of disease in cows with suspected bovine spongiform encephalopathy.
most common method for detecting cases of bovine spongiform encephalopathy (BSE). Improvement of clinical diagnosis and decision-making remains crucial. A comparison of clinical patterns, consisting of ...
Real-time PCR for simultaneous detection and genotyping of bovine viral diarrhea virus.
Publication Type: Peer reviewed scientific article Authors: Letellier, C; Pierre Kerkhofs Source: J Virol Methods, Volume 114, Issue 1, p.21-7 (2003) Keywords: Animals Bovine Virus Diarrhea-Mucosal ...
Dual labeling of Pseudomonas putida with fluorescent proteins for in situ monitoring of conjugal transfer of the TOL plasmid.
without cultivation. This method worked well for in situ monitoring of horizontal gene transfer in addition to tracking the fate of microorganisms released into complex environments. To the best of our ...
Influence of the growth substrate on the mycolic acid profiles of mycobacteria.
as an identification method of actinomycetes. Health Topics: Animal health Category: A1 FOS Classification: 4.03 Veterinary science ...
A PCR test to identify bacillus subtilis and closely related species and its application to the monitoring of wastewater biotreatment.
extracts had poor sensitivity. When assayed on cell suspensions derived from enriched wastewater samples, sensitivity was increased. Using a simple calibration method, it was possible to estimate the ...
Disinfection of wooden structures contaminated with Paenibacillus larvae subsp. larvae spores.
Paenibacillus larvae subsp. larvae spores, in order to find a practical decontamination method for hive materials. METHODS AND RESULTS: The number of viable spores recovered after the treatment, on the surface by ...
Classical swine fever virus: a second ring test to evaluate RT-PCR detection methods.
non-nested RT- PCR. The results of the laboratories were compared with one another, and with those obtained earlier when similar samples were tested by the same laboratories using non-standardised methods ...