Search results - 149 results

Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis.

adult cattle were tested. Individual test sensitivities (Se) and specificities (Sp) were estimated. Threshold assessment using the receiver operating characteristic method was also performed. The most ...

A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.

in chicken work, identifying circumstances where its use might provide misleading results. Methods for eliminating DNA contamination of RNA reduced, but did not completely remove, detectable DNA. We ...

Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli.

Source: J Virol Methods, Volume 232, p.8-11 (2016) Keywords: Animals Chromatography, Gel Epitopes Escherichia coli Influenza A virus Influenza Vaccines Levivirus Orthomyxoviridae Infections Vaccines, ...

Multiyear Serological Surveillance of Notifiable Influenza A Viruses in Belgian Poultry: A Retrospective Analysis.

ELISA has been used as first screening method since 2010. A retrospective analysis of the serological monitoring performed from 2007 through 2013 showed sporadic circulation of notifiable ...

Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance.

validation principles and methods of the World Organisation for Animal Health. Critical parameters for validation and quality control (quality metrics) are suggested, with reference to established validation ...

Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies.

Hoffmann, Bernd; Batten, Carrie Anne; Zientara, Stéphan; Bréard, Emmanuel; Van der Stede, Yves Source: J Virol Methods, Volume 228, p.140-50 (2016) Keywords: Animals Antibodies, Viral Bluetongue Bluetongue ...

A trend analysis of antimicrobial resistance in commensal Escherichia coli from several livestock species in Belgium (2011-2014).

regression and Generalized Estimating Equation and after p value adjustment for multiple testing (Linear step-up method), statistically significant decreasing temporal trends were observed for several of the ...

Fast and sensitive LC-MS/MS method measuring human mycotoxin exposure using biomarkers in urine.

/ MS method was developed to measure biomarkers for mycotoxin exposure in human urine. In total, 32 biomarkers were quantitatively or semi-quantitatively measured in 32 urine samples of Belgian ...

Evaluation of convenient pretreatment protocols for RNA virus metagenomics in serum and tissue samples.

Publication Type: Peer reviewed scientific article Authors: Rosseel, Toon; Ozhelvaci, Orkun; Freimanis, Graham; Van Borm, Steven Source: J Virol Methods, Volume 222, p.72-80 (2015) Keywords: Animals ...

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