Search results - 36 results
Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens.
Using molecular techniques, the VP2 gene was detected in various organs, providing a method to monitor vaccine uptake. rHVT- IBD vaccination induced CMI responses in specific pathogen-free chickens at ...
Towards the development of innovative multi-mycotoxin reference materials as promising metrological tool for emerging and regulated mycotoxin analyses.
Keywords: Animals Chromatography, Liquid Food Analysis Mycotoxins Reference Standards Tandem Mass Spectrometry Temperature Abstract: The interest in LC- MS / MS multi-mycotoxin methods unveiled an urgent need ...
Laboratory validation of two real-time RT-PCR methods with 5′-tailed primers for an enhanced detection of foot-and-mouth disease virus
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; David Lefebvre; Ilse De Leeuw; Steven Van Borm; Kris De Clercq Source: Journal of Virological Methods, Volume 246 ...
Investigation of a Possible Link Between Vaccination and the 2010 Sheep Pox Epizootic in Morocco.
phylogenetic analyses undertaken as part of this study showed significant differences between field isolates and the Moroccan vaccine strain. New PCR methods were developed to differentiate between wild-type ...
Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis.
adult cattle were tested. Individual test sensitivities (Se) and specificities (Sp) were estimated. Threshold assessment using the receiver operating characteristic method was also performed. The most ...
A Versatile Panel of Reference Gene Assays for the Measurement of Chicken mRNA by Quantitative PCR.
in chicken work, identifying circumstances where its use might provide misleading results. Methods for eliminating DNA contamination of RNA reduced, but did not completely remove, detectable DNA. We ...
Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli.
Source: J Virol Methods, Volume 232, p.8-11 (2016) Keywords: Animals Chromatography, Gel Epitopes Escherichia coli Influenza A virus Influenza Vaccines Levivirus Orthomyxoviridae Infections Vaccines, ...
Multiyear Serological Surveillance of Notifiable Influenza A Viruses in Belgian Poultry: A Retrospective Analysis.
ELISA has been used as first screening method since 2010. A retrospective analysis of the serological monitoring performed from 2007 through 2013 showed sporadic circulation of notifiable ...
Next-generation sequencing workflows in veterinary infection biology: towards validation and quality assurance.
validation principles and methods of the World Organisation for Animal Health. Critical parameters for validation and quality control (quality metrics) are suggested, with reference to established validation ...
Inter-laboratory evaluation of the performance parameters of a Lateral Flow Test device for the detection of Bluetongue virus-specific antibodies.
Hoffmann, Bernd; Batten, Carrie Anne; Zientara, Stéphan; Bréard, Emmanuel; Van der Stede, Yves Source: J Virol Methods, Volume 228, p.140-50 (2016) Keywords: Animals Antibodies, Viral Bluetongue Bluetongue ...