Search results - 63 results

Laboratory validation of two real-time RT-PCR methods with 5'-tailed primers for an enhanced detection of foot-and-mouth disease virus.

Publication Type: Peer reviewed scientific article Authors: VandenBussche, Frank; David Lefebvre; Ilse De Leeuw; Van Borm, Steven; Kris De Clercq Source: J Virol Methods, Volume 246, p.90-94 (2017) ...

A duplex real-time RT-PCR for the detection of bluetongue virus in bovine semen.

Publication Type: Peer reviewed scientific article Authors: Vanbinst, Tine; VandenBussche, Frank; Dernelle, Eric; Kris De Clercq Source: J Virol Methods, Volume 169, Issue 1, p.162-8 (2010) ...

Alien invasive birds.

species in Europe. For each of these alien bird species, the geographic range (native and introduced range), the introduction pathway, the general impacts and the management methods are presented. Health ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

Publication Type: Peer reviewed scientific article Authors: VandenBussche, Frank; Vandemeulebroucke, Elise; Kris De Clercq Source: Methods Mol Biol, Volume 630, p.97-108 (2010) Keywords: Animals ...

A proposed validation method for automated nucleic acid extraction and RT-qPCR analysis: an example using Bluetongue virus.

Publication Type: Peer reviewed scientific article Authors: Vandemeulebroucke, Elise; Kris De Clercq; Van der Stede, Yves; VandenBussche, Frank Source: J Virol Methods, Volume 165, Issue 1, p.76-82 ...

Evaluation of different strategies for the use of ELISA tests as first screening tools for serologic surveillance of low pathogenic avian influenza in the Belgian poultry sector.

detection of H5 and H7 subtypes. However, as the number of tested sera has been increasing, there is a need for another serologic method that could be used as a preliminary screening test. A comparison of ...

Evaluation of rapid antigen detection kits for the diagnosis of highly pathogenic avian influenza H5N1 infection.

a result of their low sensitivity and some aspecific reactions, test results always need to be confirmed by other methods. Health Topics:  Animal health Category:  A1 FOS Classification:  4.03 Veterinary ...

The most likely time and place of introduction of BTV8 into Belgian ruminants.

in 2007 and 2008. However, the origin and the time and place of the original introduction have not yet been determined. METHODS AND PRINCIPAL FINDINGS: Four retrospective epidemiological surveys have ...

Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.

Labeling Abstract: A flow cytometric method for the identification of chicken blood leukocyte subpopulations and thrombocytes was developed. An anti-chicken CD45 phycoerythrin-labelled antibody was used to ...

Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision.

Publication Type: Peer reviewed scientific article Authors: Goris, Nesya; Frank Vandenbussche; Herr, Cécile; Villers, Jérôme; Yves Van der Stede; Kris De Clercq Source: J Virol Methods, Volume 160, ...

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