Search results - 95 results
Electron tomography of negatively stained complex viruses: application in their diagnosis.
Abstract: BACKGROUND: Electron tomographic analysis can be combined with the simple and rapid negative staining technique used in electron microscopy based virus diagnosis. METHODS: Standard negative staining ...
Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability.
average coefficient of variation for repeated measurements in identical samples ranged between 4.2 and 9.7%. In conclusion, this is the first flow cytometric method suited for the simultaneous ...
Brucella suis identification and biovar typing by real-time PCR.
pathogenic for humans while others are not. Since classical biovar typing methods are often time-consuming, hard to standardize and require high-level biosafety containment, methodological improvements are ...
Comparative studies on the pathogenicity and tissue distribution of three virulence variants of classical swine fever virus, two field isolates and one vaccine strain, with special regard to immunohistochemical investigations.
swine fever virus (CSFV) and to investigate the applicability of various conventional diagnostic procedures. METHODS: 64 pigs were divided into three groups and infected with the highly virulent isolate ...
Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.
Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; Vanbinst, T; Vandemeulebroucke, E; Goris, N; Sailleau, C; Zientara, S; Kris De Clercq Source: J Virol Methods, Volume ...
Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.
sequencing of PCR-amplified, denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments, which was used as a fast screening method. The general results of the survey showed that ...
Avian influenza outbreak management: action at time of confirmation, depopulation and disposal methods; the 'Belgian experience' during the H7N7 highly pathogenic avian influenza epidemic in 2003.
risks. In the framework of the European legislation, a number of methods are authorized for the killing of poultry for processing prior to marketing. However, during emergencies such as a disease outbreak, ...
Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs.
Diseases urease Abstract: A new cultivation method was successfully applied for the in vitro isolation of a hitherto uncultured spiral Helicobacter species associated with ulceration of the non-glandular ...
Use of continuous results to compare ELISAs for the detection of antibodies to non-structural proteins of foot-and-mouth disease virus.
calculating and subsequently analysing the receiver-operator characteristic (ROC) curves and likelihood ratios (LR) for each test method. For the purposes of these analyses, test results for a total of 1337 ...
Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.
Publication Type: Peer reviewed scientific article Authors: Le Dimna, M; Vrancken, R; F. Koenen; Bougeard, S; Mesplède, A; Hutet, E; Kuntz-Simon, G; Le Potier, M F Source: J Virol Methods, Volume ...