Search results - 95 results

Electron tomography of negatively stained complex viruses: application in their diagnosis.

Abstract: BACKGROUND: Electron tomographic analysis can be combined with the simple and rapid negative staining technique used in electron microscopy based virus diagnosis. METHODS: Standard negative staining ...

Technical note: Flow cytometric identification of bovine milk neutrophils and simultaneous quantification of their viability.

average coefficient of variation for repeated measurements in identical samples ranged between 4.2 and 9.7%. In conclusion, this is the first flow cytometric method suited for the simultaneous ...

Brucella suis identification and biovar typing by real-time PCR.

pathogenic for humans while others are not. Since classical biovar typing methods are often time-consuming, hard to standardize and require high-level biosafety containment, methodological improvements are ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

Publication Type: Peer reviewed scientific article Authors: Frank Vandenbussche; Vanbinst, T; Vandemeulebroucke, E; Goris, N; Sailleau, C; Zientara, S; Kris De Clercq Source: J Virol Methods, Volume ...

Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants.

sequencing of PCR-amplified, denaturing gradient gel electrophoresis (DGGE)-separated 18S rRNA gene fragments, which was used as a fast screening method. The general results of the survey showed that ...

Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs.

Diseases urease Abstract: A new cultivation method was successfully applied for the in vitro isolation of a hitherto uncultured spiral Helicobacter species associated with ulceration of the non-glandular ...

Use of continuous results to compare ELISAs for the detection of antibodies to non-structural proteins of foot-and-mouth disease virus.

calculating and subsequently analysing the receiver-operator characteristic (ROC) curves and likelihood ratios (LR) for each test method. For the purposes of these analyses, test results for a total of 1337 ...

Validation of two commercial real-time RT-PCR kits for rapid and specific diagnosis of classical swine fever virus.

Publication Type: Peer reviewed scientific article Authors: Le Dimna, M; Vrancken, R; F. Koenen; Bougeard, S; Mesplède, A; Hutet, E; Kuntz-Simon, G; Le Potier, M F Source: J Virol Methods, Volume ...

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