Search results - 95 results

Estimating the probability of freedom of classical swine fever virus of the East-Belgium wild-boar population.

however, is not science based. We propose an alternative method to provide evidence for a wild-boar population being free of CSF and evaluate the efficiency of a surveillance programme that was implemented ...

The diversity of Mycoplasma hyopneumoniae within and between herds using pulsed-field gel electrophoresis.

enzyme, the resulting restriction profiles were clustered using the unweighted pair group method with arithmetic means (UPGMA). For both obtained dendrograms, the included isolates of the related M. ...

Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31.

lineage are important target cells in PCV2-infected pigs, but the method of binding and entry of PCV2 into these cells is unknown. Therefore, binding and entry of PCV2 to the porcine monocytic cell line 3D4 ...

Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species.

restriction analysis (ARDRA) for the identification of all 116 acknowledged Mycoplasma species and subspecies. METHODS: Based upon available 16S rDNA sequences, we calculated and compared theoretical ARDRA ...

Real Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus.

Publication Type: Peer reviewed scientific article Authors: Boxus, M; Letellier, C; Pierre Kerkhofs Source: J Virol Methods, Volume 125, Issue 2, p.125-30 (2005) Keywords: Animals Cattle Respiratory ...

Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons.

a culture-independent fingerprinting method based on PCR combined with denaturing gradient gel electrophoresis (DGGE) was developed. New PCR primers were selected which specifically targeted the 16S rRNA genes of ...

Acinetobacter diversity in environmental samples assessed by 16S rRNA gene PCR-DGGE fingerprinting.

a 100-fold decrease in sensitivity. Using a nested PCR approach, the detection limit could be lowered to at least 10 cells g(-1) of soil. The method was applied to assess Acinetobacter diversity in soil ...

Analytical performance of several classical swine fever laboratory diagnostic techniques on live animals for detection of infection.

Publication Type: Peer reviewed scientific article Authors: Dewulf, Jeroen; F. Koenen; Mintiens, Koen; Denis, Pierre; Ribbens, Stefaan; de Kruif, Aart Source: J Virol Methods, Volume 119, Issue 2, ...

Immuno-quantitative polymerase chain reaction for detection and quantitation of prion protein.

Immunosorbent Assay Humans polymerase chain reaction Prions Sensitivity and Specificity Abstract: Immuno-polymerase chain reaction (PCR) is an extremely sensitive detection method, combining the specificity of ...

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