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The origin of biased sequence depth in sequence-independent nucleic acid amplification and optimization for efficient massive parallel sequencing.
a 3’ random part used to prime complementary DNA synthesis and a 5’ defined tag sequence for subsequent amplification. Recently, this amplification method was combined with next generation sequencing to ...
In vitro and in vivo infectious potential of coxiella burnetii: a study on Belgian livestock isolates.
in the Netherlands during the 2007-2010 outbreaks. Infective doses were established with ethidium monoazide- PCR for the first time here applied to C. burnetii. This method allowed for the preparation ...
Validation of qualitative qPCR methods
Sectie Voeding (2013) Keywords: analysi analysis food Food Analysis method methods qPCR qualitative trend trends VALIDATION Health Topics: Food consumption and food safety Consommation et sécurité ...
A proteomics approach for the quantification of Staphylococcus aureus enterotoxins a and b in several matrices using uplc-msms analysis.
developed and are validating method to specifically quantify SE s in pork meat using stable isotope dilution and UPLC – MSMS analysis. Pork meat was spiked with SEA and/or SEB. Next we extract the SE s from ...
New techniques and methods developed by the Belgian NRL‐GMO to identify unauthorized GMOs in the UGMMONITOR project
Publication Type: Non-peer reviewed scientific publication Authors: Source: LabInfo, AFSCA, Belgium (2013) Keywords: New techniques and methods developed by the Belgian NRL‐GMO to identify ...
Intake and dietary sources of haem and non-haem iron among European adolescents and their association with iron status and different lifestyle and socio-economic factors.
sources and; (2) to evaluate the relation of iron intake and status in European adolescents. SUBJECTS / METHODS: Two non-consecutive 24-h recalls were completed by a computerised tool. The socio-demographic ...
MLVA as a tool for public health surveillance of human Salmonella Typhimurium: prospective study in Belgium and evaluation of MLVA loci stability.
demonstrates that MLVA improves public health surveillance of S. Typhimurium. However, the 5-loci MLVA should be complemented with other subtyping methods for investigation of possible outbreaks with frequent ...
Routinely-collected general practice data from the electronic patient record and general practitioner active electronic questioning method: A comparative study
practitioners GP healthcare Hypertension improve index INFORMATION IS IT journal Mass Mass index method methods Network networks observed Paper parameters past Patient patients Practice practitioner Practitioners ...
End-of-life communication: a retrospective survey of representative general practitioner networks in four countries
method methods Netherlands Network networks objectives OCCUPATIONAL Occupational Health older ON Patient patients practitioner prevalence PRIORITIES Priority public result results SOCIAL Spain study survey ...
End-of-life decisions: a cross-national study of treatment preference discussions and surrogate decision-maker appointments
Logistic-regression medical method methods Netherlands Network networks OCCUPATIONAL Occupational Health Participation Patient patients Planning prevalence public regression Research Research Support result results SB- ...