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Development of an NGS-Based Workflow for Improved Monitoring of Circulating Plasmids in Support of Risk Assessment of Antimicrobial Resistance Gene Dissemination.

view of AMR gene localization, i.e., chromosomal or on a plasmid. Whole-genome and plasmid DNA extraction methods were compared, as were assemblies consisting of short reads (Illumina MiSeq), long reads ...

A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.

outbreak depends on the rapid and accurate identification of the responsible food source. Conventional methods based on isolation of the pathogen from the food matrix and target-specific real-time polymerase ...

Use of Whole Genome Sequencing Data for a First in Silico Specificity Evaluation of the RT-qPCR Assays Used for SARS-CoV-2 Detection.

December 2019. COVID-19 cases are confirmed by the detection of SARS-CoV-2 RNA in biological samples by RT-qPCR. However, limited numbers of SARS-CoV-2 genomes were available when the first RT-qPCR methods ...

The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.

WGS whole genome sequencing Abstract: Gradually, conventional methods for foodborne pathogen typing are replaced by whole genome sequencing (WGS). Despite studies describing the overall benefits, ...

The use of alternative testing methods within PPP requirements

Publication Type: Scientific poster, presentation or proceeding Authors: Ph. Castelain Source: Belgian PARERE network–30.07.2020 Brussels –Teleconference (2020) Keywords: alternative testing methods ...

How to Annotate and Submit a Short Linear Motif to the Eukaryotic Linear Motif Resource.

Holger; Gibson, Toby J Source: Methods Mol Biol, Volume 2141 (2020) Abstract: Over the past few years, it has become apparent that approximately 35% of the human proteome consists of intrinsically ...

Bepaling van spoorelementen in diverse matrices met spectrometische technieken.

elaboration of (new) legislations for products on the Belgian (or European) market. Method During the bachelor thesis we worked on 2 projects regarding the determination of trace elements: 1. Food enzyme ...

The Belgian health examination survey: objectives, design and methods.

recruitment method and the methodological choices that were made in the BELHES. Methods: After a pilot period during the first quarter of the BHIS fieldwork, eligible BHIS participants were invited to ...

Benchmarking food environment policies for the prevention of diet-related non-communicable diseases in Kenya: National expert panel's assessment and priority recommendations.

policies in Kenya and identify priorities for future action. METHODS: Using the Healthy Food Environment Policy Index (Food- EPI) we collected evidence on the extent of government action to create healthy ...

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