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A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

Dominique Rousset; Steven Van Gucht *; Nancy Roosens * Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex RT-qPCR Zika ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

Source: Microbiologyopen (2019) Keywords: Luminex multiplex Public Health Surveillance sequencing Shigella Abstract: The phylogenetically closely related Shigella species and enteroinvasive Escherichia coli ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

format. In short, 200 µl of a positively flagged MGIT culture is spiked with a specific concentration of a drug, while a control sample is incubated in absence of the drug. Multiplex mRNA quantification is ...

A multiplex oligonucleotide ligation-PCR method using the Luminex technology for the geno-serotyping of the most common Salmonella in Belgium

Sophie Bertrand Source: ECCMID, ESCMID, Madrid, Spain (2018) Keywords: Luminex method multiplex Salmonella Serotyping Full text language:  English Category:  C31 FOS Classification:  3.03 Health sciences ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this study a Luminex ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of ...

Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health

a valuable alternative. In this PhD work, different molecular tools were developed, from simplex to multiplex, to detect and identify indoor airborne fungi. The goal was to improve the detection of fungal ...

Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides

developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...

The performance of multiplex immunoassays for antibody determination to diphtheria, tetanus and pertussis: a need for standardisation

Publication Type: Peer reviewed scientific article Authors: Raïssa Nadège Caboré; Denis Piérard; K Huygen Source: Vaccines, Volume 7 (2016) Keywords: a antibodies Antibody Diphtheria Immunoassay need performance pertussis Tetanus Abstract:. Service:  Immu ...

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