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A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses

Dominique Rousset; Steven Van Gucht *; Nancy Roosens * Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex RT-qPCR Zika ...

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.

a multiplex reverse transcription real-time polymerase chain reaction TaqMan® method was designed to detect universally Zika and chikungunya viruses. DESIGN: Two methods, targeting different genome segments, ...

Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

Source: Microbiologyopen (2019) Keywords: Luminex multiplex Public Health Surveillance sequencing Shigella Abstract: The phylogenetically closely related Shigella species and enteroinvasive Escherichia coli ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this study a Luminex ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for characterization of ...

Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides

developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...

The performance of multiplex immunoassays for antibody determination to diphtheria, tetanus and pertussis: a need for standardisation

Publication Type: Peer reviewed scientific article Authors: Raïssa Nadège Caboré; Denis Piérard; K Huygen Source: Vaccines, Volume 7 (2016) Keywords: a antibodies Antibody Diphtheria Immunoassay need performance pertussis Tetanus Abstract:. Service:  Immu ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

Gram-Negative Bacteria Humans Multiplex Polymerase Chain Reaction RNA, Ribosomal, 16S Abstract: OBJECTIVES: The objective of this study was to design and validate a genotyping method for multiplex identification ...

Optimized MOL-PCR for Characterization of Microbial Pathogens

surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation- PCR (MOL- PCR) optimized for characterization of microbial pathogens. With MOL- PCR, ...

A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium

foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation- PCR (MOL- PCR) assay was developed which combines ...

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