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Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.
Microbiologyopen (2019) Keywords: Luminex multiplex Public Health Surveillance sequencing Shigella Abstract: The phylogenetically closely related Shigella species and enteroinvasive Escherichia coli (EIEC) are ...
Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.
dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this ...
Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).
Biol, Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for ...
Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides
developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...
The performance of multiplex immunoassays for antibody determination to diphtheria, tetanus and pertussis: a need for standardisation
Publication Type: Peer reviewed scientific article Authors: Raïssa Nadège Caboré; Denis Piérard; K Huygen Source: Vaccines, Volume 7 (2016) Keywords: a antibodies Antibody Diphtheria Immunoassay need performance pertussis Tetanus Abstract:. Service: Immu ...
Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.
Techniques Gram-Negative Bacteria Humans Multiplex Polymerase Chain Reaction RNA, Ribosomal, 16S Abstract: OBJECTIVES: The objective of this study was to design and validate a genotyping method for multiplex ...
Optimized MOL-PCR for Characterization of Microbial Pathogens
surveillance, outbreak detection, and tracing of outbreak sources. This unit describes a multiplex oligonucleotide ligation- PCR (MOL- PCR) optimized for characterization of microbial pathogens. With MOL- PCR, ...
A multiplex oligonucleotide ligation-PCR as a complementary tool for subtyping of Salmonella Typhimurium
foodborne infections. In an attempt to overcome the intrinsic shortcomings of currently used subtyping techniques, a multiplex oligonucleotide ligation- PCR (MOL- PCR) assay was developed which combines ...
Guidelines for optimisation of a multiplex oligonucleotide ligation-PCR for characterisation of microbial pathogens in a microsphere suspension array.
p.790170 (2015) Keywords: DNA Primers DNA, Bacterial Humans Microspheres Multiplex Polymerase Chain Reaction Oligonucleotide Probes Salmonella typhimurium Abstract: With multiplex oligonucleotide ligation- ...
Isolation and genotyping of viable Toxoplasma gondii from sheep and goats in Ethiopia destined for human consumption34073
Isolation journal Laboratories Marker Markers method methods mice multiplex PCR objectives ON p PCR present regional report Research Research Support result results SB- IM Science Sheep State strain study ...