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Monitoring of human coronaviruses in Belgian primary care and hospitals, 2015-20: a surveillance study.
their hospital stay. All samples were analysed by multiplex quantitative RT- PCR s for respiratory viruses, including seasonal hCoVs OC43, NL63, and 229E. We estimated the prevalence and incidence of ...
Whole Genome Sequencing Provides an Added Value to the Investigation of Staphylococcal Food Poisoning Outbreaks
of S. aureus isolates, including some belonging to a confirmed foodborne outbreak, its added value as the ultimate multiplexing method was demonstrated. In contrast to PCR-based se gene detection for ...
Evaluating SARS-CoV-2 spike and nucleocapsid proteins as targets for antibody detection in severe and mild COVID-19 cases using a Luminex bead-based assay.
(e.g. virus neutralization assays), or are expensive with suboptimal specificity (e.g. commercial ELISA s and RDT s). Bead-based assays offer a cost-effective alternative and allow for multiplexing to ...
Development of a multiplex mass spectrometry method for simultaneous quantification of urinary proteins related to respiratory health
Publication Type: Peer reviewed scientific article Authors: S. Nauwelaerts; Nancy Roosens; Bernard, Alfred; Sigrid C.J. De Keersmaecker; Koen De Cremer Source: Scientific Reports, Volume 11, Issue 1 (2021) Manuscript versions: DOI: https://doi.org/10.10 ...
Seroprevalence of Antibodies against Diphtheria, Tetanus and Pertussis in Adult At-Risk Patients.
of antibodies against diphtheria, tetanus and pertussis to evaluate whether current vaccination programs in Belgium are adequate. Antibody titers were assessed with a bead-based multiplex assay in ...
A multiplex oligonucleotide ligation-PCR method for the genoserotyping of common Salmonella using a liquid bead suspension assay
Publication Type: Peer reviewed scientific article Authors: Mathieu Gand; Wesley Mattheus; Nancy Roosens; Katelijne Dierick; Marchal, Kathleen; Sigrid C.J. De Keersmaecker; Sophie Bertrand Source: Food Microbiology, Volume 87 (2020) Health Topics: Salmon ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
Dominique Rousset; Steven Van Gucht; Nancy Roosens Source: International Journal of Infectious Diseases, Volume 92 (2020) Keywords: Chikungunya virus discrimination identification multiplex RT-qPCR Zika virus ...
A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses.
worldwide a wave of concern. To treat patients in an early stage and prevent diffusion of the outbreak, an early diagnosis, and thus fast and adequate detection, is needed. To this aim, a multiplex reverse ...
Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.
Microbiologyopen (2019) Keywords: Luminex multiplex Public Health Surveillance sequencing Shigella Abstract: The phylogenetically closely related Shigella species and enteroinvasive Escherichia coli (EIEC) are ...
Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.
dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this ...