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Towards a Pathogenic Escherichia coli Detection Platform Using Multiplex SYBR(R)Green Real-Time PCR Methods and High Resolution Melting Analysis418
permitting the multiplex application in order to minimize the need of material and to allow for high-throughput analysis. In addition, High Resolution Melting (HRM) analysis allowing the discrimination among ...
Detection of C. botulinum neurotoxins A and B in milk by ELISA and immuno-PCR at higher sensitivity than mouse bio-assay.36466
toxin Abstract: Using commercially available antibodies and toxoids as templates, an ELISA, immuno-quantitative PCR (iqPCR), and multiplex immuno- PCR (iPCR) were developed for detection of Clostridium ...
Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.
virology Abstract: A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous ...
Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species.
methods described during the last 20 years to identify Brucella, multiplexed amplification methods offer the cheapest and simplest technical solution for molecular identification. However, one disadvantage ...
Toxoplasma gondii and Neospora caninum in wildlife: common parasites in Belgian foxes and Cervidae?
bred deer samples positive for N. caninum. Brain samples from wild roe deer, red deer and red foxes (Vulpes vulpes) were tested for the presence of T. gondii and N. caninum DNA using multiplex real-time ...
Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.
RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...
New approaches in GMO detection399
multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMO s authorized for ...
Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 136839
Flemish food Gastroenteritis genetics Humans i im Institute IS isolation & purification IT journal methods Multiplex assay Norovirus observed PCR real time PCR Real-time PCR Research Research Support ...
Prevalence of enterotoxigenic Escherichia coli virulence genes from scouring piglets in Zimbabwe.
piggeries in Zimbabwe. We used multiplex PCR for screening ST a, ST b, LT, and Stx-2e toxins. Subsequently F4, F5, F6, F18 and F41 fimbriae genes were screened in toxin positive isolates. Toxin positive ...
Distribution and evolution of ferripyoverdine receptors in Pseudomonas aeruginosa31132
Proteins microbiologie Molecular multiplex PCR ON Opportunistic Infections ORIGIN p PCR Phylogeny polymerase chain reaction present production proportion protein Proteins Pseudomonas Pseudomonas aeruginosa ...