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Development of a multiplex immunoassay for simultaneous detection of antigens in DTaP vaccines

that could be implemented for routine potency assessment of vaccines. Our work focused on the development of a Luminex-based multiplex assay (four to six-plex assay depending on the number of pertussis ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

format. In short, 200 µl of a positively flagged MGIT culture is spiked with a specific concentration of a drug, while a control sample is incubated in absence of the drug. Multiplex mRNA quantification is ...

A multiplex oligonucleotide ligation-PCR method using the Luminex technology for the geno-serotyping of the most common Salmonella in Belgium

Keersmaecker; Sophie Bertrand Source: ECCMID, ESCMID, Madrid, Spain (2018) Keywords: Luminex method multiplex Salmonella Serotyping Full text language:  English Category:  C31 FOS Classification:  3.03 Health ...

Development of xMAP and xTAG Luminex assays for public health surveillance

Publication Type: Scientific poster, presentation or proceeding Authors: Sigrid C.J. De Keersmaecker Source: xMAP® CONNECT 2016- Sharing Multiplex Solutions, NA, Issue Luminex Coorporation, NA ...

Development of a multiplex liquid bead array-based detection system for genetic characterization of pathogenic E. coli 4869

Publication Type: Scientific poster, presentation or proceeding Authors: E. Barbau-Piednoir; N Botteldoorn; Dierckx,D.; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Nineteenth Conference on Food Microbiology, NA, Issue =Belgian Society for Food Micr ...

Development of a molecular subtyping method for Salmonella Typhimurium combining different types of markers in a Luminex xTAG assay

Diseases Foodborne Diseases gene Genes Genome identification identify IS Luminex Marker Markers method Molecular molecular subtyping Multiplex assay ON outbreak outbreaks Phage type present profile Profiles ...

Molecular subtyping of Salmonella Typhimurium combining different types of markers in a multiplex liquid bead suspension array

Design detection disease Diseases Foodborne Diseases gene Genes Genome identification identify International IS Luminex method molecular subtyping Multiplex assay ON outbreak Phage type profile resistance ...

Design of multiplex assays for molecular subtyping of pathogens with the Luminex xMAP technology

IT limitation Limitations Luminex microorganism microorganisms Molecular molecular subtyping Multiplex assay Oligonucleotides outbreak outbreaks pathogen reducing regulation Sample SOFTWARE specificity ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

is incubated in absence of the drug. Multiplex mRNA quantification is performed directly on crude cell lysates using a combination of the bead-based MagPixTM (Luminex) and QuantigeneTM Plex (Thermo ...

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