Did you mean
multiple

Search results - 8 results

Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus.

virology Abstract: A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous ...

Nucleotide polymorphism-based single-tube test for robust molecular identification of all currently described Brucella species.

methods described during the last 20 years to identify Brucella, multiplexed amplification methods offer the cheapest and simplest technical solution for molecular identification. However, one disadvantage ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...

Prevalence of enterotoxigenic Escherichia coli virulence genes from scouring piglets in Zimbabwe.

piggeries in Zimbabwe. We used multiplex PCR for screening ST a, ST b, LT, and Stx-2e toxins. Subsequently F4, F5, F6, F18 and F41 fimbriae genes were screened in toxin positive isolates. Toxin positive ...

Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.

Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...

Dogs as carriers of the emerging pathogen Arcobacter.

of the emerging pathogen Arcobacter. Isolates, obtained by an Arcobacter selective isolation procedure, were identified with an Arcobacter species-specific multiplex- PCR and characterized by modified ...

A multiplex PCR to identify porcine mycoplasmas present in broth cultures.

clinical samples using the same media. We have developed a multiplex PCR as a helpful tool for rapid differentiation of these three species in the course of isolation. Based on the 16S ribosomal DNA ...

QR code

QR code for this page URL