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Shifting national surveillance of Shigella infections toward geno-serotyping by the development of a tailored Luminex assay and NGS workflow.

Microbiologyopen (2019) Keywords: Luminex multiplex Public Health Surveillance sequencing Shigella Abstract: The phylogenetically closely related Shigella species and enteroinvasive Escherichia coli (EIEC) are ...

RNA-based drug susceptibility testing of Mycobacterium tuberculosis

format. In short, 200 µl of a positively flagged MGIT culture is spiked with a specific concentration of a drug, while a control sample is incubated in absence of the drug. Multiplex mRNA quantification is ...

A multiplex oligonucleotide ligation-PCR method using the Luminex technology for the geno-serotyping of the most common Salmonella in Belgium

Keersmaecker; Sophie Bertrand Source: ECCMID, ESCMID, Madrid, Spain (2018) Keywords: Luminex method multiplex Salmonella Serotyping Full text language:  English Category:  C31 FOS Classification:  3.03 Health ...

Development and performance assessment of a luminex xMAP® direct hybridization assay for the detection and identification of indoor air fungal contamination.

dead and uncultivable fraction. Moreover, culture-based protocols are often time-consuming. In this context, molecular tools are a powerful alternative, especially those allowing multiplexing. In this ...

Molecular Subtyping of Salmonella Typhimurium with Multiplex Oligonucleotide Ligation-PCR (MOL-PCR).

Biol, Volume 1616, p.39-69 (2017) Abstract: A multiplex oligonucleotide ligation- PCR (MOL- PCR) assay is a valuable high-throughput technique for the detection of bacteria and viruses, for ...

Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health

a valuable alternative. In this PhD work, different molecular tools were developed, from simplex to multiplex, to detect and identify indoor airborne fungi. The goal was to improve the detection of fungal ...

Proof-of-concept study of a new LC-ESI-MS/MS-based assay to identify Aspergillus spp. in artificially mixed samples using species/genus-specific proteotypic peptides

developed assay which can resolve most of these MALDI- TOF MS limitations. This assay is based upon multiplex liquid chromatography electrospray ionization tandem mass spectrometry (LC- ESI- MS / MS) for the ...

The performance of multiplex immunoassays for antibody determination to diphtheria, tetanus and pertussis: a need for standardisation

Publication Type: Peer reviewed scientific article Authors: Raïssa Nadège Caboré; Denis Piérard; K Huygen Source: Vaccines, Volume 7 (2016) Keywords: a antibodies Antibody Diphtheria Immunoassay need performance pertussis Tetanus Abstract:. Service:  Immu ...

Development of a Luminex xTAG® assay for cost-effective multiplex detection of β-lactamases in Gram-negative bacteria.

Techniques Gram-Negative Bacteria Humans Multiplex Polymerase Chain Reaction RNA, Ribosomal, 16S Abstract: OBJECTIVES: The objective of this study was to design and validate a genotyping method for multiplex ...

Molecular subtyping of Salmonella Typhimurium with multiplex oligonucleotide ligation-PCR (MOL-PCR)

subtyping Multiplex assay ON pathogen PCR PRODUCTS protocol S Salmonella Salmonella enterica Salmonella typhimurium Subtyping Technique Type Viruses Abstract: A multiplex oligonucleotide ligation- PCR (MOL- ...

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