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Toxoplasma gondii and Neospora caninum in wildlife: common parasites in Belgian foxes and Cervidae?

bred deer samples positive for N. caninum. Brain samples from wild roe deer, red deer and red foxes (Vulpes vulpes) were tested for the presence of T. gondii and N. caninum DNA using multiplex real-time ...

Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR.

RT-qPCR failure due to poor sample quality, improper nucleic acid extraction and/or PCR inhibition. To increase the diagnostic capacity and reduce costs, it is recommended to use a multiplex strategy which ...

New approaches in GMO detection399

multiplexing, allowing only a few targets to be analysed at once, no longer meet the testing requirements. Along with new analytical technologies, new approaches for the detection of GMO s authorized for ...

Multiplex real-time RT-PCR for simultaneous detection of GI/GII noroviruses and murine norovirus 136839

Flemish food Gastroenteritis genetics Humans i im Institute IS isolation & purification IT journal methods Multiplex assay Norovirus observed PCR real time PCR Real-time PCR Research Research Support ...

Prevalence of enterotoxigenic Escherichia coli virulence genes from scouring piglets in Zimbabwe.

piggeries in Zimbabwe. We used multiplex PCR for screening ST a, ST b, LT, and Stx-2e toxins. Subsequently F4, F5, F6, F18 and F41 fimbriae genes were screened in toxin positive isolates. Toxin positive ...

Distribution and evolution of ferripyoverdine receptors in Pseudomonas aeruginosa31132

journal Membrane Proteins microbiologie Molecular multiplex PCR ON Opportunistic Infections ORIGIN p PCR Phylogeny polymerase chain reaction present production proportion protein Proteins Pseudomonas ...

Prevaccination distribution of human papillomavirus types in women attending at cervical cancer screening in Belgium.

and participating in cervical cancer screening, were assessed cytologically and virologically with a multiplex real-time PCR using primers targeting the E6 / E7 genes of 16 HPV types. Correlations of ...

Comparison of classical serotyping and PremiTest assay for routine identification of common Salmonella enterica serovars.

Salmonella kit uses a multiplexed DNA typing test aimed at identifying common serovars of Salmonella enterica. It was used in assays over a 9-month period in the Belgian reference laboratory that performs the ...

Effect of pooling and multiplexing on the detection of bluetongue virus RNA by real-time RT-PCR.

(RT-qPCR) was used routinely for laboratory diagnosis during the 2006/2007 bluetongue virus (BTV) serotype 8 epidemic. In the present study the impact of pooling and multiplexing strategies on RT-qPCR are ...

Dogs as carriers of the emerging pathogen Arcobacter.

of the emerging pathogen Arcobacter. Isolates, obtained by an Arcobacter selective isolation procedure, were identified with an Arcobacter species-specific multiplex- PCR and characterized by modified ...

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