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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
each other, and also to the results obtained with conventional methods. Results: A commercial kit was selected and optimized for the extraction of sufficient high-molecular weight DNA, to match the needs ...
MALDI-TOF for the identification of Mycobacteria and their drug resistance profiles
method is more challenging due to the special need for inactivation and protein extraction. Although several studies have been published, there is no clear consensus on the processing of Mycobacteria for ...
Towards a central registry for rare disease patients in Belgium
implementation improve incidence Indicator Indicators IS IT LEVEL measure measures methodology Monitoring need needs Nomenclature ON orphan Orphanet Patient Patient Care patients Planning prevalence public Quality ...
RNA-based drug susceptibility testing of Mycobacterium tuberculosis
resistance strains for the selected drugs. Conclusion We present a robust, RNA-based DST without the need for RNA extraction. The assay was proven to be efficient for isoniazid. With a total of 8 biomarker ...
Investigation of the Polycyclic Aromatic Hydrocarbons contamination in spices and dried herbs available on the Belgian market
food category for the Belgian market. Hence, there was a need to validate a method to quantify these compounds and investigate to check the compliance of spices and dried herbs available on the ...