- Did you mean
- need extraction
Search results - 24 results
Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling
resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
Keywords: DNA extraction HMW DNA Long-read Metagenomics Method evaluation nanopore Abstract: Background: Oxford Nanopore Technologies (ONT) offers an accessible platform for long-read sequencing, which ...
Inequalities in mortality associated with housing conditions in Belgium between 1991 and 2020
if the population of the whole country faced the mortality rates experienced in areas that are least deprived in terms of housing. METHODS: We used individual-level mortality data extracted from the ...
Shotgun metagenomics as a ONE Health tool for better protecting human health
each other, and also to the results obtained with conventional methods. Results: A commercial kit was selected and optimized for the extraction of sufficient high-molecular weight DNA, to match the needs ...
Development of a “Freeze-Pour” Sample Preparation Method for the GC Analysis of Semivolatile Flavouring Chemicals Present in E-cigarette Refill Liquids
comply with the notification requirements of the EU Tobacco Product Directive, the EU member state regulatory authorities need information about the exact composition of the e-liquids and their emissions. ...
Optimization and validation of an analytical method for the quantification of short- and medium-chained chlorinated paraffins in food by gas chromatography-mass spectrometry
ECNI / MS). A dispersive solid phase extraction (dSPE) method was optimized for fish, meat, oil, milk and whole-grain cereal followed by clean-up with concentrated sulfuric acid and acid silica. ...
Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?
genetic variation within the population. The aim of this study was to evaluate the characteristics and genotyping results of DNA extracted from 2 human DNA sources, selected for their rapid and noninvasive ...
DETERMINATION OF ALLERGENIC FRAGRANCE INGREDIENTS IN FEMININE ABSORBENT HYGIENE PRODUCTS AND LUBRICANTS
A liquid-liquid extraction was used as a sample ‘clean-up’ for lubricants. The sample was then injected using gas chromatography-flame ionization-mass spectrometry (GC- FID- MS). The AHP s were analyzed using a HS- ...
MALDI-TOF for the identification of Mycobacteria and their drug resistance profiles
method is more challenging due to the special need for inactivation and protein extraction. Although several studies have been published, there is no clear consensus on the processing of Mycobacteria for ...
Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health
contaminants, including the “unknown” fraction, as compared to the currently used classical monitoring methods. The necessary air sampling and DNA extraction protocols, adapted to the downstream molecular ...