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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

resolved. Shotgun metagenomics is a sequencing method involving the analysis of the genetic material directly extracted from a sample, without the need for an isolation of the bacteria. By sequencing and ...

Inequalities in mortality associated with housing conditions in Belgium between 1991 and 2020

if the population of the whole country faced the mortality rates experienced in areas that are least deprived in terms of housing. METHODS: We used individual-level mortality data extracted from the ...

Shotgun metagenomics as a ONE Health tool for better protecting human health

each other, and also to the results obtained with conventional methods. Results: A commercial kit was selected and optimized for the extraction of sufficient high-molecular weight DNA, to match the needs ...

Development of a “Freeze-Pour” Sample Preparation Method for the GC Analysis of Semivolatile Flavouring Chemicals Present in E-cigarette Refill Liquids

comply with the notification requirements of the EU Tobacco Product Directive, the EU member state regulatory authorities need information about the exact composition of the e-liquids and their emissions. ...

Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?

genetic variation within the population. The aim of this study was to evaluate the characteristics and genotyping results of DNA extracted from 2 human DNA sources, selected for their rapid and noninvasive ...

DETERMINATION OF ALLERGENIC FRAGRANCE INGREDIENTS IN FEMININE ABSORBENT HYGIENE PRODUCTS AND LUBRICANTS

A liquid-liquid extraction was used as a sample ‘clean-up’ for lubricants. The sample was then injected using gas chromatography-flame ionization-mass spectrometry (GC- FID- MS). The AHP s were analyzed using a HS- ...

MALDI-TOF for the identification of Mycobacteria and their drug resistance profiles

method is more challenging due to the special need for inactivation and protein extraction. Although several studies have been published, there is no clear consensus on the processing of Mycobacteria for ...

Development of molecular tools for rapid detection and quantification of indoor airborne molds to assess their impact on public health

contaminants, including the “unknown” fraction, as compared to the currently used classical monitoring methods. The necessary air sampling and DNA extraction protocols, adapted to the downstream molecular ...

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