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Immunogenicity and protective efficacy of tuberculosis DNA vaccines combining mycolyl-transferase Ag85A and phosphate transport receptor PstS-3.

protein was comparable following combination or single DNA vaccination. Antigenic competition was also observed at the antibody level; PstS-3 specific levels being lower in mice vaccinated with the fusion ...

Protection of rabbits against enteropathogenic Escherichia coli (EPEC) using an intimin null mutant.

a homologous, but virulent, strain was observed. The attenuated strain was able to persist in the intestinal lumen, where it elicited an immune response against EPEC-related virulence proteins, as was shown using ...

Functional characterization of opioid receptor ligands by aequorin luminescence-based calcium assay4399

Cell Cell Line Change Changes chemistry Cho Cells Cricetinae Cricetulus data Endorphins Functional Ileum im IS journal Laboratories LEVEL levels Ligands Luminescent Agents medical metabolism observed ...

Differential inlA and inlB expression and interaction with human intestinal and liver cells by Listeria monocytogenes strains of different origins36774

& purification IT journal LEVEL levels Listeria Listeria monocytogenes Liver mechanism Membrane Proteins microbiology Molecular Sequence Data mRNA Observation observed ON ORIGIN pathogenicity ...

Iron-binding antioxidant capacity is impaired in diabetes mellitus100

Observation p Patient patients physiology physiopathology plasma Print protein Proteins Research Research Support Role SB- IM serum status Transferrin Type type 2 diabetes Universities university Abstract: ...

Partial reconstitution of the CD4+-T-cell compartment in CD4 gene knockout mice restores responses to tuberculosis DNA vaccines.

PstS-3, known to encode a dominant D(b)-restricted CD8 +-T-cell epitope, displayed CD8 +-T-cell responses not observed in CD4-/- mice. M. tuberculosis challenge in reconstituted mice led to the ...

A multiplex PCR to identify porcine mycoplasmas present in broth cultures.

amplification products observed of 1129 bp (M. hyorhinis), 1000 bp (M. hyopneumoniae) and 754 bp (M. flocculare) were clearly distinguishable on a 1% agarose gel. In addition, no cross-reaction with Mycoplasma ...

Absence of viral envelope proteins in equine herpesvirus 1-infected blood mononuclear cells during cell-associated viremia.

expression of viral envelope proteins is absent, simply because the PBMC are in an early phase of infection. The percentage of infected PBMC showing surface expression of MHC-I was similar as observed in ...

A novel, highly selective inhibitor of pestivirus replication that targets the viral RNA-dependent RNA polymerase.

synthesis (EC50 = 0.12 +/- 0.02 microM) and production of infectious virus (EC50 = 0.074 +/- 0.003 microM) were observed. The selectivity index (ratio of 50% cytostatic concentration/ EC50) of BPIP was ...

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