Search results - 8 results
Transforming Shiga toxin-producing surveillance through whole genome sequencing in food safety practices.
Abstract: INTRODUCTION: Shiga toxin-producing (STEC) is a gastrointestinal pathogen causing foodborne outbreaks. Whole Genome Sequencing (WGS) in STEC surveillance holds promise in outbreak prevention and ...
Validation strategy of a bioinformatics whole genome sequencing workflow for Shiga toxin-producing Escherichia coli using a reference collection extensively characterized with conventional methods
bacterial pathogenic isolates at single nucleotide resolution, making it the ultimate tool for routine surveillance and outbreak investigation. The lack of standardization, and the variation regarding ...
Strain-Level Metagenomic Data Analysis of Enriched In Vitro and In Silico Spiked Food Samples: Paving the Way towards a Culture-Free Foodborne Outbreak Investigation Using STEC as a Case Study.
Keersmaecker Source: Int J Mol Sci, Volume 21, Issue 16 (2020) Keywords: foodborne outbreak investigation public health strain-level metagenomics Abstract: Culture-independent diagnostics, such as metagenomic ...
A Practical Method to Implement Strain-Level Metagenomics-Based Foodborne Outbreak Investigation and Source Tracking in Routine.
Sigrid C.J. De Keersmaecker Source: Microorganisms, Volume 8, Issue 8 (2020) Keywords: food surveillance Metagenomics outbreak SNP analysis STEC whole genome Abstract: The management of a foodborne ...
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country.
Vanneste; Nancy Roosens; Sigrid C.J. De Keersmaecker Source: Foods, Volume 9, Issue 8 (2020) Keywords: Food Safety foodborne outbreak investigation Shiga toxin-producing Escherichia coli STEC Surveillance WGS ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates
C.J. De Keersmaecker Source: Scientific Reports, Volume 10, Issue 1 (2020) Keywords: DNA extraction kits outbreak investigation Shiga toxin producing Escherichia coli (STEC) Whole genome sequencing (WGS) ...
Global and regional source attribution of Shiga toxin-producing Escherichia coli infections using analysis of outbreak surveillance data
Publication Type: Peer reviewed scientific article Authors: Sara M. Pires; Shannon Majowicz; Alexander Gill; Brecht Devleesschauwer Source: Epidemiology and Infection, Volume 147 (2019) Health Topics: Pathogene E. coli Pathogenic E. coli E. coli pathogèn ...
Impact of DNA extraction on whole genome sequencing analysis for characterization and relatedness of Shiga toxin-producing Escherichia coli isolates.
laboratories is essential to support foodborne pathogen control, including outbreak investigation. This study evaluated eight commercial DNA preparation kits for their potential influence on: (i) DNA quality for ...