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Assessment of the clinical and analytical performance of the Aptima SARS-CoV-2 assay using the VALCOR protocol.
public health, the performance characteristics of assays should be evaluated to ensure reliable diagnostic outcomes are achieved. METHODS: VALCOR or “ VAL idation of SARS- COR ona Virus-2 assays” is ...
HPV-based Cervical Cancer Screening on Self-samples in the Netherlands: Challenges to Reach Women and Test Performance Questions.
before testing. In the first 2 years of the new program, only 7% of screening tests were performed on a self-sample. Those who chose self-sampling versus clinician sampling were more likely to have never ...
Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence.
transmitted by ticks. Serological screenings in animals are performed to estimate the prevalence and distribution of TBEV. Most screenings consist of a primary screening by ELISA, followed by confirmation of ...
Development of strain-level shotgun metagenomics approaches to detect and characterize microbiological contaminants in the context of food safety
water, the DNA was extracted with two classical commercial kits or with a kit performing depletion of the host DNA. The extracted DNA also was amplified or not using Phi29 polymerase. We showed that all ...
IPVS policy statement on HPV nucleic acid testing guidance for those utilising/considering HPV as primary precancer screening: Quality assurance and quality control issues.
POLICY Quality Control Uterine Cervical Neoplasms Abstract: We advise that only clinically validated HPV assays which have fulfilled internationally accepted performance criteria be used for primary ...
The global burden of neglected zoonotic diseases: Current state of evidence
whereas no estimates were found for West Nile, Marburg and Lassa fever. Geographically, the highest number of studies was performed in the Netherlands (11), China (5) and Iran (4). The number of BoD ...
Comparison of 6 DNA extraction methods for isolation of high yield of high molecular weight DNA suitable for shotgun metagenomics Nanopore sequencing to detect bacteriaAbstractBackgroundResultsConclusion
evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for ...
Development of a Taxon-Specific Real-Time Polymerase Chain Reaction Method to Detect Trichoderma reesei Contaminations in Fermentation Products
method was assessed as specific and sensitive (LOD95 % = eight copies). In addition, the developed real-time PCR TR method performance was confirmed to be in line with the “Minimum Performance Requirements ...
Burden of infectious disease studies in Europe and the United Kingdom: a review of methodological design choices
databases and grey literature were searched for articles reporting the assessment of DALY and its components. We considered studies in which researchers performed DALY calculations using primary ...
Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants
Publication Type: Peer reviewed scientific article Authors: Laura Van Poelvoorde *; François Dufrasne *; Steven Van Gucht *; Xavier Saelens; Nancy Roosens * Source: Current Issues in Molecular Biology, Volume 45, Issue 3 (2023) Health Topics: Health and ...