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Effect of challenge dose and age in experimental infection of pigs with encephalomyocarditis virus.
Swine Diseases Abstract: Two experiments were performed to compare the severity of encephalomyocarditis virus (EMCV) infection in pigs. The pigs were challenged with the Greek myocardial strain, at ...
Evaluation of virulence of Mycoplasma hyopneumoniae field isolates.
hyopneumoniae. RAPD analysis was performed on all M. hyopneumoniae strains. Significant differences between isolates were found for the RDS, lung lesion score, histopathology, immunofluorescence and serology. ...
Carbon source-induced modifications in the mycolic acid content and cell wall permeability of Rhodococcus erythropolis E1.
estimated by high-performance liquid chromatography and mass spectrometry. Two types of mycolic acid patterns were observed after growth either on saturated linear alkanes or on short-chain alkanoates. One ...
Influence of the growth substrate on the mycolic acid profiles of mycobacteria.
Mycobacterium spp. LB501T, LB307T and VM552 was examined by high-performance liquid chromatography (HPLC) using glucose, alkanes, polycyclic aromatic hydrocarbons (PAH) or Luria-Bertani medium (LB) as the sole ...
Transmission of encephalomyocarditis virus (EMCV) among pigs experimentally quantified.
transmission experiments were performed to estimate the basic reproduction ratio R(0), indicating the level of encephalomyocarditis virus (EMCV) transmission among pigs. In a first experimental set-up with nine ...
Characterization of monoclonal antibodies directed against the bovine herpesvirus-1 glycoprotein E and use for the differentiation between vaccinated and infected animals.
glycoprotein and one on the gE/gI complex. Blocking experiments were performed with sera from experimentally vaccinated and infected cattle. A competition was observed between gE-positive bovine sera and six of ...
First evidence of Johne's disease in farmed red deer (Cervus elaphus) in Belgium.
negative. Direct and indirect tests were performed on 24 hinds and stags (yearlings, 2- and 4-year-old animals). The indirect tests performed were serology (Mycobacterium paratuberculosis antibody ELISA, ...
Production of antibodies against chicken interferon-gamma: demonstration of neutralizing activity and development of a quantitative ELISA.
addition to being more rapid and easier to perform than classical cell-mediated immunity tests, this ELISA has excellent sensitivity and improved specificity. The use of a specific rabbit polyclonal serum as ...
Classical swine fever virus: a ring test to evaluate RT-PCR detection methods.
false positive results. Several of the PCR tests appear suitable in terms of specificity and sensitivity. Further trials are necessary to compare results when the same test is performed by different ...
Long-term protection against bovine leukaemia virus replication in cattle and sheep.
viruses and naked DNA injections are able to delay BLV infection, although complete protection cannot be achieved. In addition, our data cast light onto the need to perform long-term vaccination trials ...