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Strain-level characterization without culture enrichment? Easing and accelerating outbreak investigation using shotgun metagenomics facilitated with nanopore adaptive sampling

adaptive sampling, following whole genome amplification to increase the amount of genetic material to meet sequencing standards. The strain-level data analysis was performed as previously described (Buytaers ...

Open Lab Application for the Characterization of Nanomaterials by Transmission Electron Microscopy

expertise and instrumentation to measure the size, morphology, crystallographic structure and chemical composition of a wide range of NM by EM. Identification and measurement of particles can be performed in ...

In vitro model to study the endocrine disrupting activity of migration products from plastic food contact materials

a recent study performed by Simoneau & al, 2012. Human estrogen receptor agonistic and antagonistic activities of 25 pure compounds were measured using MCF7- ER cells (genetically modified MCF7 cells). ...

An innovative integrated approach based on DNA walking to identify unauthorized GMOs

family vector, frequently present in transgenic plants. The presented integrated approach is performed in two main successive steps on Bt rice grains. First, the potential presence of unauthorized GMO s is ...

Detection of Staphyloccocus aureus enterotoxin B using online SPE-UPLC-MSMS

aureus ultra-performance liquid chromatography-tandem mass spectrometry Health Topics:  Food consumption and food safety Consommation et sécurité alimentaires Voedselconsumptie en –veiligheid Service:  ...

Identification of potential migrants from printing inks used on paper and board food contact materials

little pieces, then 40 mL acetonitrile was added and the migration was performed at 70°C for 24h. Afterwards, the extracts were analysed using gas chromatography-mass spectrometry (GC- MS n) in full scan ...

The occurrence and formation of benzene in foodstuffs

dietary exposure for the adult Belgium population was performed. In addition, since several reactions in foods may give rise to the generation of hydroxyl radicals, their potential to decarboxylate benzoate ...

An Alternative Method Validation Strategy for the European Decision 2002/657/EC

ISO 11843 standards are used to set up an experimental validation plan that minimizes the number of samples without increasing the uncertainty of the performance characteristics. At each level (0.5, ...

Food contamination with pyrrolizidine alkaloids: the expected and the unexpected

food items.   The Belgian Scientific Institute of Public Health (WIV- ISP) has directed a project aiming to report occurrence levels of PA s and PANO s in targeted food items to perform a dietary exposure ...

UHPLC-MS/MS methods for the quantification of pyrrolizidine alkaloids in a wide range of food matrices and exposure assessment for the Belgian population

PANO s in a large range of food items.   The Belgian Scientific Institute of Public Health has directed a project aiming to report occurrence levels of PA s and PANO s in targeted food items to perform ...

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